highly divergent Encephalomyocarditis virus isolated from nonhuman primates in Singapore

• Published in: Virology journal Vol. 10; no. 1; p. 248
• Main Authors: Yeo, Dawn Su-Yin, Lian, Jing Er, Fernandez, Charlene J, Lin, Yueh-Nuo, Liaw, Jasper Chin-Wen, Soh, Moi-Lien, Lim, Elizabeth Ai-Sim, Chan, Kwai-Peng, Ng, Mah-Lee, Tan, Hwee-Cheng, Oh, Serena, Ooi, Eng-Eong, Tan, Boon-Huan
• Format: Journal Article
• Language: English
• Published: England Springer-Verlag 02.08.2013; BioMed Central Ltd; BioMed Central
• Subjects: adults; amino acids; Analysis; Animals; Animals, Zoo; Antibodies; antiserum; cell culture; Cercopithecus aethiops; Cluster Analysis; Diagnosis; electron microscopy; Encephalomyocarditis virus; Encephalomyocarditis virus - classification; Encephalomyocarditis virus - genetics; Encephalomyocarditis virus - isolation & purification; etiological agents; gene targeting; genes; Genome, Viral; Genomics; Health aspects; heart; Heart - virology; host range; juveniles; Lung - virology; Microscopy, Electron, Transmission; Molecular Sequence Data; Myocarditis; neutralizing antibodies; Phylogeny; Physiological aspects; Picornaviruses; Pongo - virology; Primates; reverse transcriptase polymerase chain reaction; RNA, Viral - genetics; Sequence Analysis, DNA; Sequence Homology; serological surveys; Singapore; tissue culture; Vero Cells; Viral Proteins - genetics; virion; Virion - ultrastructure; Virus Cultivation; viruses; zoo animals; Zoos; Singapore

BACKGROUND: In 2001 and 2002, fatal myocarditis resulted in the sudden deaths of four, two adult and two juvenile, orang utans out of a cohort of 26 in the Singapore Zoological Gardens. METHODS: Of the four orang utans that underwent post-mortem examination, virus isolation was performed from the ti...