Two-amino acids change in the nsp4 of SARS coronavirus abolishes viral replication

• Published in: Virology (New York, N.Y.) Vol. 510; pp. 165 - 174
• Main Authors: Sakai, Yusuke, Kawachi, Kengo, Terada, Yutaka, Omori, Hiroko, Matsuura, Yoshiharu, Kamitani, Wataru
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.10.2017
• Subjects: Amino Acid Substitution; amino acids; cell membranes; complementary DNA; Coronavirus; DNA Mutational Analysis; genome; messenger RNA; Nsp4; Protein Interaction Mapping; proteins; replicon; SARS Virus - genetics; SARS Virus - physiology; Severe acute respiratory syndrome; Severe acute respiratory syndrome-related coronavirus; transcription (genetics); Viral Nonstructural Proteins - genetics; Viral replication; Virus Replication; Coronavirus; Nsp4; Viral replication; Severe acute respiratory syndrome

Infection with coronavirus rearranges the host cell membrane to assemble a replication/transcription complex in which replication of the viral genome and transcription of viral mRNA occur. Although coexistence of nsp3 and nsp4 is known to cause membrane rearrangement, the mechanisms underlying the i...