Autophagy inhibition promotes paclitaxel-induced apoptosis in cancer cells

• Published in: Cancer letters Vol. 307; no. 2; pp. 141 - 148
• Main Authors: Xi, Guangmin, Hu, Xiaoyan, Wu, Baolin, Jiang, Hanming, Young, Charles Y.F., Pang, Yingxin, Yuan, Huiqing
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 28.08.2011; Elsevier Limited
• Subjects: Antibiotics; Antineoplastic Agents, Phytogenic; Antineoplastic Agents, Phytogenic - pharmacology; Apoptosis; Apoptosis - drug effects; Autophagy; Autophagy - drug effects; Blotting, Western; Cancer; Cancer therapies; Cell Line, Tumor; Chemotherapy; Cytotoxicity; drug effects; genes; green fluorescent protein; Hematology, Oncology and Palliative Medicine; Humans; Lung cancer; organelles; Paclitaxel; Paclitaxel - pharmacology; pharmacology; Proteins; RNA Interference; RNA, Small Interfering; small interfering RNA; Lung cancer; AVOs; Paclitaxel; 3-MA; MTT; LC3; Autophagy; Apoptosis; microtubule-associated protein 1 light chain 3; acidic vesicular organelles; 3-methyladenine; 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazoliumbromide

Paclitaxel has been demonstrated to be an effective mitotic inhibitor and apoptosis inducer to treat aggressive malignancies. In this paper, we have provided a line of evidence that promotion of apoptotic cell death by paclitaxel was accompanied with induction of autophagy in A549 cells. Paclitaxel...