Zebrafish High-Throughput Screening to Study the Impact of Dissolvable Metal Oxide Nanoparticles on the Hatching Enzyme, ZHE1

• Published in: Small (Weinheim an der Bergstrasse, Germany) Vol. 9; no. 9-10; pp. 1776 - 1785
• Main Authors: Lin, Sijie, Zhao, Yan, Ji, Zhaoxia, Ear, Jason, Chang, Chong Hyun, Zhang, Haiyuan, Low-Kam, Cecile, Yamada, Kristin, Meng, Huan, Wang, Xiang, Liu, Rong, Pokhrel, Suman, Mädler, Lutz, Damoiseaux, Robert, Xia, Tian, Godwin, Hilary A., Lin, Shuo, Nel, André E.
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 27.05.2013; WILEY‐VCH Verlag; Wiley Subscription Services, Inc
• Subjects: Amino Acid Sequence; Animals; COPPER OXIDE; CUPRIC OXIDE; Danio rerio; Embryos; Enzymes; Freshwater; hatching interference; high throughput screening; High-Throughput Screening Assays; IMPACT PROPERTIES; Interference; Metal Nanoparticles - chemistry; Metal Nanoparticles - toxicity; Metal oxides; Metalloproteases - metabolism; Molecular Sequence Data; Nanoparticles; Nanotechnology; OXIDES; Oxides - chemistry; PARTICLES; predictive toxicology; Proteins; ROBOTS; Screening; Solubility; Zebrafish; Zebrafish - embryology

The zebrafish is emerging as a model organism for the safety assessment and hazard ranking of engineered nanomaterials. In this Communication, the implementation of a roboticized high‐throughput screening (HTS) platform with automated image analysis is demonstrated to assess the impact of dissolvabl...