Microfabricated elastomeric stencils for micropatterning cell cultures

• Published in: Journal of biomedical materials research Vol. 52; no. 2; p. 346
• Main Authors: Folch, A, Jo, B H, Hurtado, O, Beebe, D J, Toner, M
• Format: Journal Article
• Language: English
• Published: United States 01.11.2000
• Subjects: Animals; Cell Adhesion; Cell Culture Techniques - instrumentation; Coated Materials, Biocompatible; Dimethylpolysiloxanes; Fibroblasts; Humans; Silicones

Here we present an inexpensive method to fabricate microscopic cellular cultures, which does not require any surface modification of the substrate prior to cell seeding. The method utilizes a reusable elastomeric stencil (i.e., a membrane containing thru holes) which seals spontaneously against the...