重度子痫前期胎盘中CD142的表达与其启动子区甲基化程度的关系
目的探究CD142在重度子痫前期孕妇胎盘中的表达情况及其与启动子区域甲基化之间的关系。方法本研究采用实时荧光定量PCR、免疫组化、蛋白质印迹检测24例重度子痫前期孕妇和24例正常妊娠孕妇胎盘中CD142基因的表达情况;采用甲基化特异性PCR检测CD142启动子区域的甲基化情况。结果病例组胎盘中CD142mRNA相对含量（1.45±0.42）高于正常妊娠孕妇（0.25±0.28）,差异有统计学意义（P〈0.05）;病例组胎盘中CD142蛋白表达量（0.857±0.043）高于正常晚孕者（0.248±0.035）,差异有统计学意义（P〈0.05）;病例组胎盘组织中CD142启动子区域的甲基化阳性率...
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| Published in | 西安交通大学学报（医学版） Vol. 38; no. 6; pp. 883 - 887 |
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| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
西安交通大学第一附属医院妇产科,陕西西安,710061%西安交通大学第一附属医院周围血管科,陕西西安,710061%西安交通大学第一附属医院乳腺外科,陕西西安,710061%山阳县医院妇产科,陕西商洛,726400
2017
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1671-8259 |
| DOI | 10.7652/jdyxb201706019 |
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| Abstract | 目的探究CD142在重度子痫前期孕妇胎盘中的表达情况及其与启动子区域甲基化之间的关系。方法本研究采用实时荧光定量PCR、免疫组化、蛋白质印迹检测24例重度子痫前期孕妇和24例正常妊娠孕妇胎盘中CD142基因的表达情况;采用甲基化特异性PCR检测CD142启动子区域的甲基化情况。结果病例组胎盘中CD142mRNA相对含量（1.45±0.42）高于正常妊娠孕妇（0.25±0.28）,差异有统计学意义（P〈0.05）;病例组胎盘中CD142蛋白表达量（0.857±0.043）高于正常晚孕者（0.248±0.035）,差异有统计学意义（P〈0.05）;病例组胎盘组织中CD142启动子区域的甲基化阳性率低于对照组（29.2%vs.100.0%,χ2=36.11,P〈0.001）,非甲基化阳性率高于正常组（100.0%vs.20.8%,χ2=29.85,P〈0.001）;CD142的蛋白表达水平与其启动子区域的甲基化程度呈负相关（r=-0.909,P〈0.05）。结论 CD142通过甲基化调控蛋白的表达在重度子痫前期的发病过程中发挥重要作用。 |
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| AbstractList | R714.24; 目的 探究CD142在重度子痫前期孕妇胎盘中的表达情况及其与启动子区域甲基化之间的关系.方法 本研究采用实时荧光定量PCR、免疫组化、蛋白质印迹检测24例重度子痫前期孕妇和24例正常妊娠孕妇胎盘中CD142基因的表达情况;采用甲基化特异性PCR检测CD142启动子区域的甲基化情况.结果病例组胎盘中CD142 mRNA相对含量(1.45±0.42)高于正常妊娠孕妇(0.25±0.28),差异有统计学意义(P<0.05);病例组胎盘中CD142蛋白表达量(0.857±0.043)高于正常晚孕者(0.248±0.035),差异有统计学意义(P<0.05);病例组胎盘组织中CD142启动子区域的甲基化阳性率低于对照组(29.2%vs.100.0%,χ2=36.11,P<0.001),非甲基化阳性率高于正常组(100.0%vs.20.8%,χ2=29.85,P<0.001);CD142的蛋白表达水平与其启动子区域的甲基化程度呈负相关(r=-0.909,P<0.05).结论 CD142 通过甲基化调控蛋白的表达在重度子痫前期的发病过程中发挥重要作用. 目的探究CD142在重度子痫前期孕妇胎盘中的表达情况及其与启动子区域甲基化之间的关系。方法本研究采用实时荧光定量PCR、免疫组化、蛋白质印迹检测24例重度子痫前期孕妇和24例正常妊娠孕妇胎盘中CD142基因的表达情况;采用甲基化特异性PCR检测CD142启动子区域的甲基化情况。结果病例组胎盘中CD142mRNA相对含量（1.45±0.42）高于正常妊娠孕妇（0.25±0.28）,差异有统计学意义（P〈0.05）;病例组胎盘中CD142蛋白表达量（0.857±0.043）高于正常晚孕者（0.248±0.035）,差异有统计学意义（P〈0.05）;病例组胎盘组织中CD142启动子区域的甲基化阳性率低于对照组（29.2%vs.100.0%,χ2=36.11,P〈0.001）,非甲基化阳性率高于正常组（100.0%vs.20.8%,χ2=29.85,P〈0.001）;CD142的蛋白表达水平与其启动子区域的甲基化程度呈负相关（r=-0.909,P〈0.05）。结论 CD142通过甲基化调控蛋白的表达在重度子痫前期的发病过程中发挥重要作用。 |
| Abstract_FL | Objective To explore the relationship between expression of CD142 protein and the promoter methylation in the placenta of severe preeclampsia patients.Methods We assessed 24 patients complicated with severe preeclampsia as case group and 24 normal pregnant women as control group via qRT-PCR, immunohistochemistry and Western blotting for CD142 expression and MSP technology for methylation in CD142 promoter region.Results The relative expression quantity of CD142 mRNA in severe preeclampsia group (1.45± 0.42)was higher than that in normal group (0.25±0.28)(P <0.05).The expression quantity of CD142 protein in severe preeclampsia group (0.857±0.043)was higher than that in normal group (0.248 ±0.035)(P <0.05).The positive rate of CD142 promoter region methylation was lower in severe preeclampsia group than in normal group (29.2% vs .100.0%,χ2 =36.11,P <0.001)while the positive rate of CD142 promoter region unmethylation was higher than that in the latter group (100.0% vs .20.8%,χ2 =29.85,P <0.001).A negative correlation was found between the CD142 promoter methylation level and the expression quantity of CD142 protein (r = -0.909,P <0.05).Conclusion The expression of CD142 protein regulated by the promoter methylation plays a crucial role in the mechanism of severe preeclampsia. |
| Author | 刘睿 马强 文安辛 田改彦 王文娟 蔺飒 王慰敏 |
| AuthorAffiliation | 西安交通大学第一附属医院妇产科,陕西西安710061 西安交通大学第一附属医院周围血管科,陕西西安710061 西安交通大学第一附属医院乳腺外科,陕西西安710061 山阳县医院妇产科,陕西商洛726400 |
| AuthorAffiliation_xml | – name: 西安交通大学第一附属医院妇产科,陕西西安,710061%西安交通大学第一附属医院周围血管科,陕西西安,710061%西安交通大学第一附属医院乳腺外科,陕西西安,710061%山阳县医院妇产科,陕西商洛,726400 |
| Author_FL | WEN An-xin LIN Sa WANG Wei-min LIU Rui MA Qiang WANG Wen-juan TIAN Gai-yan |
| Author_FL_xml | – sequence: 1 fullname: LIU Rui – sequence: 2 fullname: MA Qiang – sequence: 3 fullname: WEN An-xin – sequence: 4 fullname: TIAN Gai-yan – sequence: 5 fullname: WANG Wen-juan – sequence: 6 fullname: LIN Sa – sequence: 7 fullname: WANG Wei-min |
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| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| DOI | 10.7652/jdyxb201706019 |
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| DocumentTitleAlternate | Relationship between expression of CD142 protein and the promoter methylation in the placenta in severe preeclampsia |
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| Keywords | CD142 启动子 methylation 重度子痫前期 placenta 胎盘 promoter 甲基化 severe preeclampsia |
| Language | Chinese |
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| Notes | LIU Rui1 , MA Qiang2 , WEN An-xina , TIAN Gai-yan1 , WANG Wen-juan1 , LIN Sa4, WANG Wei-min1 （1. Department of Gynecology and Obstetrics; 2. Department of Peripheral Vessels; 3. Department of Breast Surgery, the First Affiliated Hospital of Xi＇an Jiaotong University, Xi＇an 710061 ; 4. Department of Gynecology and Obstetrics, Shanyang County Hospital, Shangluo 726400, China） severe preeclampsia ; CD142 ; promoter; methylation; placenta Objective To explore the relationship between expression of CD142 protein and the promoter methylation in the placenta of severe preeclampsia patients.Methods We assessed 24 patients complicated with severe preeclampsia as case group and 24 normal pregnant women as control group via qRT-PCR,immunohistochemistry and Western blotting for CD142 expression and MSP technology for methylation in CD142 promoter region.Results The relative expression quantity of CD142 mRNA in severe preeclampsia group（1.45±0.42）was higher than that in normal group（0.25±0.28）（P〈0.05）.The expression quantit |
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| Publisher | 西安交通大学第一附属医院妇产科,陕西西安,710061%西安交通大学第一附属医院周围血管科,陕西西安,710061%西安交通大学第一附属医院乳腺外科,陕西西安,710061%山阳县医院妇产科,陕西商洛,726400 |
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| Snippet | ... R714.24; 目的 探究CD142在重度子痫前期孕妇胎盘中的表达情况及其与启动子区域甲基化之间的关系.方法... |
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| SubjectTerms | CD142 启动子 甲基化 胎盘 重度子痫前期 |
| Title | 重度子痫前期胎盘中CD142的表达与其启动子区甲基化程度的关系 |
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