Multiple-Locus Variable-Number Tandem-Repeat Analysis of Mycoplasma pneumoniae Isolates between 2004 and 2014 in Yamagata, Japan: Change in Molecular Characteristics during an 11-year Period

• Published in: Japanese Journal of Infectious Diseases Vol. 70; no. 6; pp. 642 - 646
• Main Authors: Shimotai, Yoshitaka, Itagaki, Tsutomu, Mizuta, Katsumi, Suzuki, Yu, Ikeda, Tatsuya, Hongo, Seiji, Seto, Junji, Matsuzaki, Yoko, Katsushima, Fumio, Katsushima, Yuriko
• Format: Journal Article
• Language: English
• Published: Japan National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee 2017; Japan Science and Technology Agency
• Subjects: DNA, Bacterial; History, 21st Century; Humans; Japan - epidemiology; Loci; macrolide resistance; Macrolides - pharmacology; Microbial Sensitivity Tests; Minisatellite Repeats; MLVA; molecular epidemiology; Multilocus Sequence Typing; Mutation; Mycoplasma pneumoniae; Mycoplasma pneumoniae - classification; Mycoplasma pneumoniae - drug effects; Mycoplasma pneumoniae - genetics; Mycoplasma pneumoniae - isolation & purification; P1 type; Pneumonia, Mycoplasma - epidemiology; Pneumonia, Mycoplasma - history; Pneumonia, Mycoplasma - microbiology; Prevalence; Public Health Surveillance; Typing; VNTR; Japan; P1 type; molecular epidemiology; MLVA; VNTR; macrolide resistance
• ISSN: 1344-6304; 1884-2836; 1884-2836
• DOI: 10.7883/yoken.JJID.2017.276

Multiple-locus variable-number tandem-repeat analysis (MLVA) typing was performed for Mycoplasma pneumoniae strains isolated between 2004 and 2014 in Yamagata, Japan. The results were examined by considering the combination of the P1 type and prevalence of macrolide resistance-associated mutations. Four-locus (Mpn13–16) MLVA classified 347 strains into 9 MLVA types, including 3 major types: 3-5-6-2, 4-5-7-2, and 4-5-7-3. All type 3-5-6-2 strains (77 strains) were P1 type 2 variants (2a or 2c), while types 4-5-7-2 (181 strains) and 4-5-7-3 (75 strains) were P1 type 1. MLVA type 4-5-7-2 strains circulated and were dominant until 2010, accounting for 88.4% of the 121 strains isolated between 2004 and 2010. The prevalence of types 4-5-7-3 and 3-5-6-2 strains increased rapidly in 2011 and 2012, respectively, resulting in cocirculation of 3 MLVA types, including type 4-5-7-2, between 2011 and 2013. The prevalence of macrolide resistance-associated mutations in MLVA types 4-5-7-2, 4-5-7-3, and 3-5-6-2 strains was 59.7% (108/181), 25.3% (19/75), and 0% (0/77), respectively. Because the prevalence of macrolide resistance-associated mutations differed by current MLVA types in Yamagata, continued surveillance combined with molecular typing and identification of macrolide resistance-associated mutations is necessary.