JMJD6 cleaves MePCE to release positive transcription elongation factor b (P-TEFb) in higher eukaryotes

• Published in: eLife Vol. 9
• Main Authors: Lee, Schuyler, Liu, Haolin, Hill, Ryan, Chen, Chunjing, Hong, Xia, Crawford, Fran, Kingsley, Molly, Zhang, Qianqian, Liu, Xinjian, Chen, Zhongzhou, Lengeling, Andreas, Bernt, Kathrin Maria, Marrack, Philippa, Kappler, John, Zhou, Qiang, Li, Chuan-Yuan, Xue, Yuhua, Hansen, Kirk, Zhang, Gongyi
• Format: Journal Article
• Language: English
• Published: England eLife Science Publications, Ltd 12.02.2020; eLife Sciences Publications Ltd; eLife Sciences Publications, Ltd
• Subjects: 7SK snRNP; Analysis; Animal models; Animals; Arginine; Binding Sites; Biochemistry and Chemical Biology; Blotting, Western; CDK9; Crystal structure; Deoxyribonucleic acid; DNA; DNA methylation; DNA-directed RNA polymerase; Enzymes; Gene Knockout Techniques; Genes; House mouse; JMJD6; Mass Spectrometry; MePCE; Messenger RNA; Methyltransferases - metabolism; Mice; Novels; P-TEFb; Phosphorylation; Positive Transcriptional Elongation Factor B - metabolism; Proteases; Protein Structure, Tertiary; Proteins; Proteolysis; Receptors, Cell Surface - chemistry; Receptors, Cell Surface - metabolism; Ribonucleoproteins (small nuclear); RNA; RNA polymerase; RNA polymerase II; RNA Polymerase II - metabolism; Transcription (Genetics); Transcription elongation factor b; CDK9; mouse; 7SK snRNP; chemical biology; MePCE; biochemistry; RNA polymerase II; JMJD6; P-TEFb

More than 30% of genes in higher eukaryotes are regulated by promoter-proximal pausing of RNA polymerase II (Pol II). Phosphorylation of Pol II CTD by positive transcription elongation factor b (P-TEFb) is a necessary precursor event that enables productive transcription elongation. The exact mechan...