Neuronal cell depolarization induces intragenic chromatin modifications affecting NCAM alternative splicing

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 106; no. 11; pp. 4325 - 4330
• Main Authors: Schor, Ignacio E, Rascovan, Nicolás, Pelisch, Federico, Alló, Mariano, Kornblihtt, Alberto R
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 17.03.2009; National Acad Sciences
• Subjects: Acetylation; Alternative Splicing; Animals; Biological Sciences; Cell adhesion & migration; Cells; Chromatin; Chromatin - genetics; Depolarization; DNA polymerase; Epigenesis, Genetic; Exons; Genes; Histones; Histones - metabolism; Kinases; Membrane Potentials - physiology; Mutation; Neural cell adhesion molecules; Neural Cell Adhesion Molecules - genetics; Neurons; Neurons - cytology; Neurons - physiology; Promoter regions; Rats; RNA Polymerase II - metabolism; Splicing

In search for physiological pathways affecting alternative splicing through its kinetic coupling with transcription, we found that membrane depolarization of neuronal cells triggers the skipping of exon 18 from the neural cell adhesion molecule (NCAM) mRNA, independently of the calcium/calmodulin pr...