Direct detection of elephant endotheliotropic herpesvirus 1 (EEHV1) DNA in heparinized plasma by loop-mediated isothermal amplification

• Published in: Journal of Veterinary Medical Science Vol. 85; no. 4; pp. 459 - 462
• Main Authors: TAKEHANA, Kazuya, MATSUNO, Keita
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 2023; Japan Science and Technology Agency; The Japanese Society of Veterinary Science
• Subjects: Animals; Asian elephants (Elephas maximus); Deoxyribonucleic acid; Diagnosis; direct detection; DNA; elephant endotheliotropic herpes virus (EEHV); Elephants; Gene amplification; Hemorrhagic disease; heparinized plasma; Herpes viruses; Herpesviridae - genetics; Herpesviridae Infections - diagnosis; Herpesviridae Infections - veterinary; loop-mediated isothermal amplification (LAMP); Viremia; Wildlife Science; Asian elephants (Elephas maximus); elephant endotheliotropic herpes virus (EEHV); heparinized plasma; loop-mediated isothermal amplification (LAMP); direct detection

Elephant endotheliotropic herpesvirus (EEHV) causes a fatal hemorrhagic disease and is a significant cause of mortality in juvenile Asian elephants. A loop-mediated isothermal amplification (LAMP) method was developed to rapidly diagnose EEHV viremia. However, extracting DNA from whole blood samples...