Microbial Biofilm Voltammetry: Direct Electrochemical Characterization of Catalytic Electrode-Attached Biofilms

• Published in: Applied and Environmental Microbiology Vol. 74; no. 23; pp. 7329 - 7337
• Main Authors: Marsili, Enrico, Rollefson, Janet B, Baron, Daniel B, Hozalski, Raymond M, Bond, Daniel R
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.12.2008; American Society for Microbiology (ASM)
• Subjects: anaerobic digesters; bacteria; biofilm; Biofilms; Biofilms - growth & development; Biological and medical sciences; Bioreactors; carbon; Carbon - metabolism; Cell growth; Cells; dielectric spectroscopy; Electricity; Electrocatalysis; Electrochemistry; Electrodes; Electrodes - microbiology; Electron transfer; Enzymes; Fundamental and applied biological sciences. Psychology; Geobacter - growth & development; Geobacter - metabolism; Geobacter sulfurreducens; immobilized enzymes; Microbiology; Physiology and Biotechnology; quantitative analysis; Studies; Voltammetry; Characterization; Electrodes; Biofilm; Electrochemistry; Voltammetry; Microorganism

While electrochemical characterization of enzymes immobilized on electrodes has become common, there is still a need for reliable quantitative methods for study of electron transfer between living cells and conductive surfaces. This work describes growth of thin (<20 μm) Geobacter sulfurreducens...