Ultra-Rapid Real-Time RT-PCR Method for Detecting Middle East Respiratory Syndrome Coronavirus Using a Mobile PCR Device, PCR1100

• Published in: Japanese Journal of Infectious Diseases Vol. 73; no. 3; pp. 181 - 186
• Main Authors: Shirato, Kazuya, Nao, Naganori, Matsuyama, Shutoku, Kageyama, Tsutomu
• Format: Journal Article
• Language: English
• Published: Japan National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee 29.05.2020; Japan Science and Technology Agency
• Subjects: Assaying; Containment; Control equipment; Coronaviruses; Diagnosis; Genetic screening; Genomes; MERS coronavirus (MERS-CoV); Middle East respiratory syndrome; Middle East Respiratory Syndrome (MERS); PCR1100; Polymerase chain reaction; Real time; Respiratory diseases; Reverse transcription; Ribonucleic acid; RNA; ultra-rapid real-time RT-PCR; Middle East; ultra-rapid real-time RT-PCR; MERS coronavirus (MERS-CoV); Middle East Respiratory Syndrome (MERS); PCR1100

Middle East respiratory syndrome (MERS) coronavirus (MERS-CoV) is usually diagnosed through highly sensitive and specific genetic tests such as real-time reverse transcription polymerase chain reaction (RT-PCR). Currently, two real-time RT-PCR assays targeting the upE and ORF1a regions of the MERS-C...