A Novel System for Constructing a Recombinant Highly-Attenuated Vaccinia Virus Strain (LC16m8) Expressing Foreign Genes and Its Application for the Generation of LC16m8-Based Vaccines against Herpes Simplex Virus 2

• Published in: Japanese Journal of Infectious Diseases Vol. 71; no. 3; pp. 229 - 233
• Main Authors: Omura, Natsumi, Yoshikawa, Tomoki, Fujii, Hikaru, Shibamura, Miho, Inagaki, Takuya, Kato, Hirofumi, Egawa, Kazutaka, Harada, Shizuko, Yamada, Souichi, Takeyama, Haruko, Saijo, Masayuki
• Format: Journal Article
• Language: English
• Published: Japan National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee 2018; Japan Science and Technology Agency
• Subjects: Dosage; Fatalities; Fluorescence; Genes; Glycoprotein B; Glycoproteins; Herpes simplex; Herpes viruses; Immunoglobulin G; LC16m8; Lethal dose; Mice; Smallpox; Survival; Vaccine; Vaccine efficacy; Vaccines; Vaccinia virus; Viruses; LC16m8; Vaccinia virus; Vaccine

A novel system was developed for generating highly attenuated vaccinia virus LC16m8 (m8, third-generation smallpox vaccine) that expresses foreign genes. The innovations in this system are its excisable selection marker, specificity of the integration site of a gene of interest, and easy identificat...