High-dimensional analysis of intestinal immune cells during helminth infection

• Published in: eLife Vol. 9
• Main Authors: Ferrer-Font, Laura, Mehta, Palak, Harmos, Phoebe, Schmidt, Alfonso J, Chappell, Sally, Price, Kylie M, Hermans, Ian F, Ronchese, Franca, le Gros, Graham, Mayer, Johannes U
• Format: Journal Article
• Language: English
• Published: England eLife Science Publications, Ltd 11.02.2020; eLife Sciences Publications Ltd; eLife Sciences Publications, Ltd
• Subjects: Animals; B cells; cell isolation; Confocal microscopy; Dimensional analysis; Duodenum; Duodenum - immunology; Duodenum - parasitology; EDTA; Experiments; Flow cytometry; Flow Cytometry - methods; H. polygyrus; Health aspects; helminth infection; Host-Parasite Interactions - immunology; Immune response; Immunology and Inflammation; Infections; intestinal immune cells; Intestine; Intestines; Isolation; Mice, Inbred C57BL; Microscopy; Nematospiroides dubius; Optimization; Parasites; Protocol; Tools and Resources; type 2 immunity; mouse; helminth infection; inflammation; cell isolation; H. polygyrus; intestinal immune cells; immunology; type 2 immunity

Single cell isolation from helminth-infected murine intestines has been notoriously difficult, due to the strong anti-parasite type 2 immune responses that drive mucus production, tissue remodeling and immune cell infiltration. Through the systematic optimization of a standard intestinal digestion p...