Addition of ascorbic acid to the extracellular environment activates lipoplexes of a ferrocenyl lipid and promotes cell transfection

• Published in: Journal of controlled release Vol. 157; no. 2; pp. 249 - 259
• Main Authors: Aytar, Burcu S., Muller, John P.E., Golan, Sharon, Hata, Shinichi, Takahashi, Hiro, Kondo, Yukishige, Talmon, Yeshayahu, Abbott, Nicholas L., Lynn, David M.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 30.01.2012; Elsevier
• Subjects: Animals; Ascorbic acid; Ascorbic Acid - administration & dosage; Ascorbic Acid - chemistry; Biological and medical sciences; cell culture; Cell Line; Cercopithecus aethiops; culture media; DNA; External stimulus; Ferrocenyl lipid; Ferrous Compounds - administration & dosage; Ferrous Compounds - chemistry; gene expression; Gene Transfer Techniques; General pharmacology; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; Humans; lipids; Lipoplexes; Luciferases - genetics; Luciferases - metabolism; Luminescent Agents; Medical sciences; Mice; nanomaterials; Nanostructure; oxidation; Oxidation-Reduction; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; reducing agents; spectroscopy; Transfection; Transgenes - genetics; transmission electron microscopy; zeta potential; Ferrocenyl lipid; Nanostructure; Transfection; Ascorbic acid; External stimulus; Lipoplexes; Stimulus; Pharmaceutical technology; Vitamin; Lipids; In vitro; Genetic transfer; Environment; Lipoplex

The level of cell transfection mediated by lipoplexes formed using the ferrocenyl lipid bis(11-ferrocenylundecyl)dimethylammonium bromide (BFDMA) depends strongly on the oxidation state of the two ferrocenyl groups of the lipid (reduced BFDMA generally mediates high levels of transfection, but oxidi...