Tetraspanins distinguish separate extracellular vesicle subpopulations in human serum and plasma – Contributions of platelet extracellular vesicles in plasma samples

• Published in: Journal of extracellular vesicles Vol. 11; no. 5; pp. e12213 - n/a
• Main Authors: Karimi, Nasibeh, Dalirfardouei, Razieh, Dias, Tomás, Lötvall, Jan, Lässer, Cecilia
• Format: Journal Article
• Language: English
• Published: United States John Wiley & Sons, Inc 01.05.2022; John Wiley and Sons Inc; Wiley
• Subjects: activated platelets; Biomarkers; Blood; Blood Platelets; CD63 antigen; CD81 antigen; cd9; CD9 antigen; Cell Biology; Cell culture; Centrifugation; Clinical Laboratory Medicine; Coagulants; Edetic acid; Edetic Acid - analysis; exosomes; Extracellular vesicles; Extracellular Vesicles - chemistry; Flow cytometry; Humans; integrin; Klinisk laboratoriemedicin; Laboratories; Lipoproteins; Mass Spectrometry; Mass spectroscopy; microvesicles; multiplex; Neurosciences; Neurovetenskaper; Plasma; Platelets; protein; Proteins; rna; secretion; serum; subpopulations; surface; Tetraspanins - analysis; United States > US; Sweden; Germany; extracellular vesicles; biomarkers; exosomes; subpopulations; microvesicles; plasma; serum

Background: The ability to isolate extracellular vesicles (EVs) from blood is vital in the development of EVs as disease biomarkers. Both serum and plasma can be used, but few studies have compared these sources in terms of the type of EVs that are obtained. The aim of this study was to determine th...