Establishing Sustainable Cell Lines of a Coral, Acropora tenuis

• Published in: Marine biotechnology (New York, N.Y.) Vol. 23; no. 3; pp. 373 - 388
• Main Authors: Kawamura, Kaz, Nishitsuji, Koki, Shoguchi, Eiichi, Fujiwara, Shigeki, Satoh, Noriyuki
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.06.2021; Springer Nature B.V
• Subjects: Acropora tenuis; Biomedical and Life Sciences; biotechnology; Blastula; Cell culture; Cell lines; Cells; Clumps; Clusters; Collagen; Collagenase; Colour; Corals; Culture media; Dissociation; ectoderm; Engineering; Epidermis; Ethylenediaminetetraacetic acids; Freshwater & Marine Ecology; Genes; Larvae; Life Sciences; Microbiology; Morphogenesis; neurons; Original; Original Article; Plasmin; Properties; Pseudopodia; sequence analysis; Skin; Trypsin; Zoology; Cell dissociation; Coral; Single-cell RNA-seq; Planula larvae; Cell lines

Planula larvae of the scleractinian coral, Acropora tenuis , consist of elongated ectodermal cells and developing inner endodermal cells. To establish in vitro cell lines for future studies of cellular and developmental potential of coral cells, larvae were successfully dissociated into single cells...