Ascorbic Acid Improves the Developmental Competence of Porcine Oocytes After Parthenogenetic Activation and Somatic Cell Nuclear Transplantation

• Published in: Journal of Reproduction and Development Vol. 59; no. 1; pp. 78 - 84
• Main Authors: SIRIBOON, Chawalit, LO, Neng-Wen, KERE, Michel, NGUYEN, Ngoc Tan, JU, Jyh-Cherng
• Format: Journal Article
• Language: English
• Published: Japan THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT 2013; The Society for Reproduction and Development
• Subjects: Animals; Antioxidants - pharmacology; Apoptosis; Ascorbic Acid - metabolism; Ascorbic Acid - pharmacology; Blastocyst - cytology; Blastocyst - physiology; Cloning, Molecular; Culture Media - metabolism; Culture system; Cytoplasm - metabolism; Dose-Response Relationship, Drug; Embryo Culture Techniques; Embryo development; Female; Glutathione - metabolism; Handmade cloning; Nuclear Transfer Techniques; Oocytes - cytology; Oocytes - physiology; Original; Parthenogenesis - physiology; Parthenote; Reactive Oxygen Species - metabolism; Swine; Vitamin C
• ISSN: 0916-8818; 1348-4400
• DOI: 10.1262/jrd.2012-114

In this study, a dose-response assessment was performed to understand the relation between supplementation of media with L-ascorbic acid or vitamin C and porcine oocyte maturation and the in vitro development of parthenotes (PA) and handmade cloned (HMC) embryos. Various concentrations (0, 25, 50 an...