Traumatic Acid Reduces Oxidative Stress and Enhances Collagen Biosynthesis in Cultured Human Skin Fibroblasts

• Published in: Lipids Vol. 51; no. 9; pp. 1021 - 1035
• Main Authors: Jabłońska-Trypuć, Agata, Pankiewicz, Walentyn, Czerpak, Romuald
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.09.2016; Springer Nature B.V
• Subjects: antioxidants; Biomedical and Life Sciences; Biosynthesis; Catalase; Cell Membrane - metabolism; Cells, Cultured; chemical structure; Collagen; Collagen - biosynthesis; culture media; Cytokinins; Dicarboxylic Acids - pharmacology; Enzymatic activity; enzyme activity; Fatty acids; fibroblasts; Fibroblasts - cytology; Fibroblasts - drug effects; Fibroblasts - metabolism; Glutathione; Glutathione peroxidase; Glutathione reductase; Humans; Life Sciences; Lipid peroxidation; Lipid Peroxidation - drug effects; Lipidology; Medical Biochemistry; Medicinal Chemistry; Microbial Genetics and Genomics; Neurochemistry; Nutrition; Original; Original Article; Oxidative stress; Oxidative Stress - drug effects; Peroxidation; Phaseolus vulgaris; phospholipids; Phospholipids - metabolism; plant hormones; secretion; Skin - cytology; Skin - drug effects; Skin diseases; thiols; Traumatic acid; unsaturated fatty acids; Glutathione peroxidase; Cytokinins; Catalase; Glutathione reductase; Collagen; Lipid peroxidation; Traumatic acid; Glutathione
• ISSN: 0024-4201; 1558-9307; 1558-9307
• DOI: 10.1007/s11745-016-4174-5

Traumatic acid (TA) is a plant hormone (cytokinin) that in terms of chemical structure belongs to the group of fatty acids derivatives. It was isolated from Phaseolus vulgaris . TA activity and its influence on human cells and organism has not previously been the subject of research. The aim of this study was to examine the effects of TA on collagen content and basic oxidative stress parameters, such as antioxidative enzyme activity, reduced glutathione, thiol group content, and lipid peroxidation in physiological conditions. The results show a stimulatory effect of TA on tested parameters. TA caused a decrease in membrane phospholipid peroxidation and exhibited protective properties against ROS production. It also increases protein and collagen biosynthesis and its secretion into the culture medium. The present findings reveal that TA exhibits multiple and complex activity in fibroblast cells in vitro . TA, with its activity similar to unsaturated fatty acids, shows antioxidant and stimulatory effects on collagen biosynthesis. It is a potentially powerful agent with applications in the treatment of many skin diseases connected with oxidative stress and collagen biosynthesis disorders.