1个新的玉米半透明皱缩胚乳突变体的遗传分析及基因定位
【目的】解析半透明皱缩玉米胚乳形成机制．【方法】以纯合玉米突变体M-E-479为供试材料，通过对M-E-479籽粒进行表型鉴定、遗传分析、主要成分测定及扫描电镜观察，构建定位群体，利用图位克隆技术对突变基因进行遗传定位．【结果和结论】M-E-479突变体胚乳中脂肪含量增加，蛋白质和淀粉含量下降，淀粉粒的大小、形状、排列空间发生改变；用F2群体将突变基因定位在10号染色体SSR标记bnlg1074与umc1506之间，两标记的遗传距离约为3.6 cM，两标记的物理距离约为2.2 Mb．可见M-E-479是一个新的玉米半透明皱缩胚乳突变体，突变性状是由隐性单基因控制．...
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| Published in | 华南农业大学学报 Vol. 35; no. 5; pp. 31 - 35 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
吉林农业大学生命科学学院，吉林长春130118
2014
吉林省农业科学院农业生物技术研究所，吉林长春130124%吉林农业大学生命科学学院,吉林长春,130118%吉林省中玉农业有限公司,吉林长春,130012%吉林省农业科学院农业生物技术研究所,吉林长春,130124 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1001-411X |
| DOI | 10.7671/j.issn.1001-411X.2014.05.006 |
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| Abstract | 【目的】解析半透明皱缩玉米胚乳形成机制．【方法】以纯合玉米突变体M-E-479为供试材料，通过对M-E-479籽粒进行表型鉴定、遗传分析、主要成分测定及扫描电镜观察，构建定位群体，利用图位克隆技术对突变基因进行遗传定位．【结果和结论】M-E-479突变体胚乳中脂肪含量增加，蛋白质和淀粉含量下降，淀粉粒的大小、形状、排列空间发生改变；用F2群体将突变基因定位在10号染色体SSR标记bnlg1074与umc1506之间，两标记的遗传距离约为3.6 cM，两标记的物理距离约为2.2 Mb．可见M-E-479是一个新的玉米半透明皱缩胚乳突变体，突变性状是由隐性单基因控制． |
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| AbstractList | 【目的】解析半透明皱缩玉米胚乳形成机制．【方法】以纯合玉米突变体M-E-479为供试材料，通过对M-E-479籽粒进行表型鉴定、遗传分析、主要成分测定及扫描电镜观察，构建定位群体，利用图位克隆技术对突变基因进行遗传定位．【结果和结论】M-E-479突变体胚乳中脂肪含量增加，蛋白质和淀粉含量下降，淀粉粒的大小、形状、排列空间发生改变；用F2群体将突变基因定位在10号染色体SSR标记bnlg1074与umc1506之间，两标记的遗传距离约为3.6 cM，两标记的物理距离约为2.2 Mb．可见M-E-479是一个新的玉米半透明皱缩胚乳突变体，突变性状是由隐性单基因控制． S513.035.2; 【目的】解析半透明皱缩玉米胚乳形成机制．【方法】以纯合玉米突变体M-E-479为供试材料，通过对M-E-479籽粒进行表型鉴定、遗传分析、主要成分测定及扫描电镜观察，构建定位群体，利用图位克隆技术对突变基因进行遗传定位．【结果和结论】M-E-479突变体胚乳中脂肪含量增加，蛋白质和淀粉含量下降，淀粉粒的大小、形状、排列空间发生改变；用F2群体将突变基因定位在10号染色体SSR标记bnlg1074与umc1506之间，两标记的遗传距离约为3.6 cM，两标记的物理距离约为2.2 Mb．可见M-E-479是一个新的玉米半透明皱缩胚乳突变体，突变性状是由隐性单基因控制． |
| Abstract_FL | Objective To analyze the formation mechanism of translucent and shrunken maize endo-sperm.[Method] The mutant M-E-479 of homozygous maize was used as experimental materials in our study .Through phenotypic identification , genetic analysis , main ingredients measurement and scanning electron microscopy , mapping population of M-E-479 was selected and map-based cloning technology was performed to locate the mutant gene .[Result and conclusion] The result showed that the amount of fat in-creased , while the protein and starch decreased , and the starch grains changed in size , shape and space configuration in the mutant endosperm of M-E-479 .The mutant gene is located between SSR markers bn-lg1074 and umc1506 of chromosome 10 by using F2 population .Meanwhile , the genetic distance is about 3.6 cM and the physical distance is about 2.2 Mb.The results suggest that M-E-479 is a new translucent and shrunken maize endosperm mutant , and its mutant character may be controlled by a recessive single gene . |
| Author | 崔喜艳 胡广宇 孙小杰 佟珊珊 陈众峰 刘相国 |
| AuthorAffiliation | 吉林农业大学生命科学学院,吉林长春130118 吉林省农业科学院农业生物技术研究所,吉林长春130124 吉林省中玉农业有限公司,吉林长春130012 |
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| Author_FL | CHEN Zhongfeng CUI Xiyan TONG Shanshan LIU Xiangguo HU Guangyu SUN Xiaojie |
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| ClassificationCodes | S513.035.2 |
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| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| DocumentTitleAlternate | A genetic analysis and gene mapping of a new translucent and shrunken endosperm mutant in maize |
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| Keywords | 胚乳突变体 遗传分析 genetic analysis 玉米 基因定位 gene mapping endosperm mutant maize |
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| Notes | 44-1110/S maize;endosperm mutant;genetic analysis;gene mapping Objective To analyze the formation mechanism of translucent and shrunken maize endo-sperm.[Method] The mutant M-E-479 of homozygous maize was used as experimental materials in our study .Through phenotypic identification , genetic analysis , main ingredients measurement and scanning electron microscopy , mapping population of M-E-479 was selected and map-based cloning technology was performed to locate the mutant gene .[Result and conclusion] The result showed that the amount of fat in-creased , while the protein and starch decreased , and the starch grains changed in size , shape and space configuration in the mutant endosperm of M-E-479 .The mutant gene is located between SSR markers bn-lg1074 and umc1506 of chromosome 10 by using F2 population .Meanwhile , the genetic distance is about 3.6 cM and the physical distance is about 2.2 Mb.The results suggest that M-E-479 is a new translucent and shrunken maize endosperm mutant , and its mutant charact |
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| PublicationTitle | 华南农业大学学报 |
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| PublicationYear | 2014 |
| Publisher | 吉林农业大学生命科学学院，吉林长春130118 吉林省农业科学院农业生物技术研究所，吉林长春130124%吉林农业大学生命科学学院,吉林长春,130118%吉林省中玉农业有限公司,吉林长春,130012%吉林省农业科学院农业生物技术研究所,吉林长春,130124 |
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| SubjectTerms | 基因定位 玉米 胚乳突变体 遗传分析 |
| Title | 1个新的玉米半透明皱缩胚乳突变体的遗传分析及基因定位 |
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