functional single-molecule binding assay via force spectroscopy

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 104; no. 40; pp. 15677 - 15681
• Main Authors: Cao, Yi, Balamurali, M.M, Sharma, Deepak, Li, Hongbin
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 02.10.2007; National Acad Sciences
• Subjects: Atomic force microscopy; Atomic interactions; Binding Sites; Biochemistry; Biological Sciences; Biotechnology; Fluorescence; Histograms; Humans; Immunoglobulin Fc Fragments - chemistry; Immunoglobulin G - chemistry; Kinetics; Ligands; Microscopy, Atomic Force; Molecules; Physical Sciences; Polyproteins; Protein Binding; Protein Denaturation; Protein Folding; Proteins; Proteins - chemistry; Receptors, GABA-B - chemistry; Receptors, GABA-B - metabolism; Spectroscopy; Spectrum analysis; Studies

Protein-ligand interactions, including protein-protein interactions, are ubiquitously essential in biological processes and also have important applications in biotechnology. A wide range of methodologies have been developed for quantitative analysis of protein-ligand interactions. However, most of...