Photoactivatable CRISPR-Cas9 for optogenetic genome editing

• Published in: Nature biotechnology Vol. 33; no. 7; pp. 755 - 760
• Main Authors: Nihongaki, Yuta, Kawano, Fuun, Nakajima, Takahiro, Sato, Moritoshi
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.07.2015; Nature Publishing Group
• Subjects: 42/41; 631/1647/1511; 631/1647/2253; 631/92/552; Agriculture; Base Sequence; Bioinformatics; Biomedical and Life Sciences; Biomedical engineering; Biomedical Engineering/Biotechnology; Biomedical research; Biomedicine; Biotechnology; Cellular biology; CRISPR-Cas Systems - genetics; CRISPR-Cas Systems - radiation effects; Genetic engineering; Genetic Engineering - methods; Genetic research; Genomics; HEK293 Cells; Humans; Irradiation; Kidneys; letter; Life Sciences; Models, Genetic; Molecular Sequence Data; Optogenetics - methods; RNA Interference

The genome editing activity of CRISPR-Cas9 can be switched on and off by light using split Cas9 fragments fused tophotoinducible dimerization domains. We describe an engineered photoactivatable Cas9 (paCas9) that enables optogenetic control of CRISPR-Cas9 genome editing in human cells. paCas9 consis...