Lithium Evokes Expression of Vegetal-Specific Molecules in the Animal Blastomeres of Sea Urchin Embryos

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 86; no. 10; pp. 3669 - 3673
• Main Authors: Livingston, Brian T., Wilt, Fred H.
• Format: Journal Article
• Language: English
• Published: Washington, DC National Academy of Sciences of the United States of America 01.05.1989; National Acad Sciences
• Subjects: Alkaline Phosphatase - metabolism; Animals; Biological and medical sciences; Blastomeres; Cell Differentiation - drug effects; Cell differentiation, maturation, development, hematopoiesis; Cell lines; Cell physiology; Cell Separation - methods; Cytoskeletal Proteins - genetics; Developmental biology; Embryonic cells; Embryos; Extracellular Matrix Proteins; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation; Histocytochemistry; In Vitro Techniques; Lithium - pharmacology; Marine; Molecular and cellular biology; Molecules; Nucleic Acid Hybridization; Phosphatases; Sea urchins; Sea Urchins - embryology; Sea water; Space life sciences; Strongylocentrotus purpuratus; Alkaline phosphatase; Echinodermata; Lithium; Echinoidea; Invertebrata; Blastomere; Cell differentiation; Gene expression; Sea urchin

The mechanism of determination of early embryonic cells has been investigated using sea urchin embryos. An efficacious method of isolating blastomere pairs from the animal or vegetal half of sea urchin embryos was developed. The overt differentiation of separated animal and vegetal blastomere pairs...