Converting nonsense codons into sense codons by targeted pseudouridylation

• Published in: Nature (London) Vol. 474; no. 7351; pp. 395 - 398
• Main Authors: Karijolich, John, Yu, Yi-Tao
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.06.2011; Nature Publishing Group
• Subjects: 631/337/1645/2570; 631/337/574; Amino Acid Sequence; Amino acids; Amino Acids - genetics; Amino Acids - metabolism; Base Sequence; Biological and medical sciences; Codon; Codon - genetics; Codon, Nonsense - genetics; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Fungal; Genetic Code - genetics; Genetic research; Genetic translation; Humanities and Social Sciences; letter; Mass spectrometry; Metallothionein - genetics; Metallothionein - metabolism; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; multidisciplinary; Peptide Chain Termination, Translational; Physiological aspects; Protein Biosynthesis - genetics; Proteins; Pseudouridine - metabolism; Ribosomes - metabolism; RNA, Fungal - genetics; RNA, Fungal - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae Proteins - genetics; Science; Science (multidisciplinary); Translation. Translation factors. Protein processing; tRNA Methyltransferases - genetics; United States; Pseudouridine; Nonsense mutation; Genetic translation; Posttranscriptional modification

Nonsense codons taken as read The three 'nonsense' or 'stop' codons that signal the ribosome to cease translation — UAA, UAG and UGA — all contain an initial uridine residue. John Karijolich and Yi-Tao Yu demonstrate that isomerization of this uridine, converting it to pseudourid...