Preserved effector functions of human ORAI1- and STIM1-deficient neutrophils

• Published in: Journal of allergy and clinical immunology Vol. 137; no. 5; pp. 1587 - 1591.e7
• Main Authors: Elling, Roland, Keller, Baerbel, Weidinger, Carl, Häffner, Monika, Deshmukh, Sachin D., Zee, Isabelle, Speckmann, Carsten, Ehl, Stephan, Schwarz, Klaus, Feske, Stefan, Henneke, Philipp
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.05.2016; Elsevier Limited
• Subjects: Allergy and Immunology; Endoplasmic reticulum; Flow cytometry; Labeling; Ligands; Lymphocytes; Mutation; Neutrophils; Patients; Stem cells
• ISSN: 0091-6749; 1097-6825; 1097-6825
• DOI: 10.1016/j.jaci.2015.09.047

[...]we compared expression levels of ORAI and STIM homologues between PMNs, B lymphocytes, and T lymphocytes by using publicly available RNA sequencing data from healthy subjects.7 ORAI1 and STIM1 were similarly expressed in PMNs and lymphocytes. [...]mRNA levels of the different ORAI and STIM homologues are comparable across leukocyte subsets (see Fig E1 in this article's Online Repository at www.jacionline.org). [...]FcγRIII (CD16) cross-linking also induced Ca2+ influx in ORAI1 p.R91W PMN that was only moderately reduced compared with that in control PMNs (see Fig E4 in this article's Online Repository at www.jacionline.org). Because cytosolic Ca2+ concentrations are considered essential for antibacterial PMN functions, we examined whether ORAI1 deficiency impaired the PMN response to various microbial and nonmicrobial ligands.