Human serum-derived protein removes the need for coating in defined human pluripotent stem cell culture

Reliable, scalable and time-efficient culture methods are required to fully realize the clinical and industrial applications of human pluripotent stem (hPS) cells. Here we present a completely defined, xeno-free medium that supports long-term propagation of hPS cells on uncoated tissue culture plast...

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Published inNature communications Vol. 7; no. 1; p. 12170
Main Authors Pijuan-Galitó, Sara, Tamm, Christoffer, Schuster, Jens, Sobol, Maria, Forsberg, Lars, Merry, Catherine L. R., Annerén, Cecilia
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 13.07.2016
Nature Publishing Group
Nature Portfolio
Subjects
101/58
13
13/1
13/100
13/106
13/51
14
14/19
14/63
38
38/22
38/39
38/77
38/90
631/136/532/2064
631/1647/1407/651
631/45/612/1221
631/80
82
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Alpha-Globulins
Cell culture
Cell Culture Techniques - methods
Cell Proliferation
Cells, Cultured
Culture Media
Human Embryonic Stem Cells - cytology
Humanities and Social Sciences
Humans
Induced Pluripotent Stem Cells - cytology
Kinases
multidisciplinary
Pluripotent Stem Cells - cytology
Proteins
Science
Science (multidisciplinary)
Stem cells
Sweden
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Abstract Reliable, scalable and time-efficient culture methods are required to fully realize the clinical and industrial applications of human pluripotent stem (hPS) cells. Here we present a completely defined, xeno-free medium that supports long-term propagation of hPS cells on uncoated tissue culture plastic. The medium consists of the Essential 8 (E8) formulation supplemented with inter-α-inhibitor (IαI), a human serum-derived protein, recently demonstrated to activate key pluripotency pathways in mouse PS cells. IαI efficiently induces attachment and long-term growth of both embryonic and induced hPS cell lines when added as a soluble protein to the medium at seeding. IαI supplementation efficiently supports adaptation of feeder-dependent hPS cells to xeno-free conditions, clonal growth as well as single-cell survival in the absence of Rho-associated kinase inhibitor (ROCKi). This time-efficient and simplified culture method paves the way for large-scale, high-throughput hPS cell culture, and will be valuable for both basic research and commercial applications. Improved culture methods are needed to reliably grow human pluripotent stem cells (hPSCs) on a large scale. Here, the authors identify a xeno-free medium with a supplement of Inter-α-inhibitor that supports long-term propagation and improved single-cell passaging of hPSCs on uncoated plastic.
AbstractList Reliable, scalable and time-efficient culture methods are required to fully realize the clinical and industrial applications of human pluripotent stem (hPS) cells. Here we present a completely defined, xeno-free medium that supports long-term propagation of hPS cells on uncoated tissue culture plastic. The medium consists of the Essential 8 (E8) formulation supplemented with inter-α-inhibitor (IαI), a human serum-derived protein, recently demonstrated to activate key pluripotency pathways in mouse PS cells. IαI efficiently induces attachment and long-term growth of both embryonic and induced hPS cell lines when added as a soluble protein to the medium at seeding. IαI supplementation efficiently supports adaptation of feeder-dependent hPS cells to xeno-free conditions, clonal growth as well as single-cell survival in the absence of Rho-associated kinase inhibitor (ROCKi). This time-efficient and simplified culture method paves the way for large-scale, high-throughput hPS cell culture, and will be valuable for both basic research and commercial applications.
Reliable, scalable and time-efficient culture methods are required to fully realize the clinical and industrial applications of human pluripotent stem (hPS) cells. Here we present a completely defined, xeno-free medium that supports long-term propagation of hPS cells on uncoated tissue culture plastic. The medium consists of the Essential 8 (E8) formulation supplemented with inter-alpha-inhibitor (I alpha I), a human serum-derived protein, recently demonstrated to activate key pluripotency pathways in mouse PS cells. IaI efficiently induces attachment and long-term growth of both embryonic and induced hPS cell lines when added as a soluble protein to the medium at seeding. IaI supplementation efficiently supports adaptation of feeder-dependent hPS cells to xeno-free conditions, clonal growth as well as single-cell survival in the absence of Rho-associated kinase inhibitor (ROCKi). This time-efficient and simplified culture method paves the way for large-scale, high-throughput hPS cell culture, and will be valuable for both basic research and commercial applications.
Reliable, scalable and time-efficient culture methods are required to fully realize the clinical and industrial applications of human pluripotent stem (hPS) cells. Here we present a completely defined, xeno-free medium that supports long-term propagation of hPS cells on uncoated tissue culture plastic. The medium consists of the Essential 8 (E8) formulation supplemented with inter-α-inhibitor (IαI), a human serum-derived protein, recently demonstrated to activate key pluripotency pathways in mouse PS cells. IαI efficiently induces attachment and long-term growth of both embryonic and induced hPS cell lines when added as a soluble protein to the medium at seeding. IαI supplementation efficiently supports adaptation of feeder-dependent hPS cells to xeno-free conditions, clonal growth as well as single-cell survival in the absence of Rho-associated kinase inhibitor (ROCKi). This time-efficient and simplified culture method paves the way for large-scale, high-throughput hPS cell culture, and will be valuable for both basic research and commercial applications. Improved culture methods are needed to reliably grow human pluripotent stem cells (hPSCs) on a large scale. Here, the authors identify a xeno-free medium with a supplement of Inter-α-inhibitor that supports long-term propagation and improved single-cell passaging of hPSCs on uncoated plastic.
Improved culture methods are needed to reliably grow human pluripotent stem cells (hPSCs) on a large scale. Here, the authors identify a xeno-free medium with a supplement of Inter-α-inhibitor that supports long-term propagation and improved single-cell passaging of hPSCs on uncoated plastic.
ArticleNumber 12170
Author Schuster, Jens
Forsberg, Lars
Tamm, Christoffer
Pijuan-Galitó, Sara
Sobol, Maria
Merry, Catherine L. R.
Annerén, Cecilia
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  givenname: Christoffer
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  surname: Schuster
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  givenname: Maria
  surname: Sobol
  fullname: Sobol, Maria
  organization: Department of Immunology, Genetics and Pathology and Science for Life Laboratory
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  givenname: Lars
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  organization: Department of Immunology, Genetics and Pathology and Science for Life Laboratory
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  givenname: Catherine L. R.
  surname: Merry
  fullname: Merry, Catherine L. R.
  organization: Department of Medical Biochemistry and Microbiology, Box 582, Uppsala University, Stem Cell Glycobiology Group, Wolfson Centre for Stem Cells
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  givenname: Cecilia
  surname: Annerén
  fullname: Annerén, Cecilia
  email: cecilia.anneren@ge.com
  organization: Department of Medical Biochemistry and Microbiology, Box 582, Uppsala University, GE Healthcare Bio-Sciences AB
BackLink https://www.ncbi.nlm.nih.gov/pubmed/27405751$$D View this record in MEDLINE/PubMed
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Snippet Reliable, scalable and time-efficient culture methods are required to fully realize the clinical and industrial applications of human pluripotent stem (hPS)...
Improved culture methods are needed to reliably grow human pluripotent stem cells (hPSCs) on a large scale. Here, the authors identify a xeno-free medium with...
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Alpha-Globulins
Cell culture
Cell Culture Techniques - methods
Cell Proliferation
Cells, Cultured
Culture Media
Human Embryonic Stem Cells - cytology
Humanities and Social Sciences
Humans
Induced Pluripotent Stem Cells - cytology
Kinases
multidisciplinary
Pluripotent Stem Cells - cytology
Proteins
Science
Science (multidisciplinary)
Stem cells
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Title Human serum-derived protein removes the need for coating in defined human pluripotent stem cell culture
URI https://link.springer.com/article/10.1038/ncomms12170
https://www.ncbi.nlm.nih.gov/pubmed/27405751
https://www.proquest.com/docview/1803463875
https://search.proquest.com/docview/1804198506
https://pubmed.ncbi.nlm.nih.gov/PMC4947164
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-302125
https://doaj.org/article/3ff4aa12a81242048dcf3aaa0077653e
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