Particle bombardment-assisted peptide-mediated gene transfer for highly efficient transient assay

• Published in: BMC research notes Vol. 16; no. 1; p. 46
• Main Authors: Kimura, Mitsuhiro, Endo, Akira, Nagira, Yozo, Yoshizumi, Takeshi
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 06.04.2023; BioMed Central; BMC
• Subjects: Centrifugation; Chloroplasts; Cytotoxicity; Deoxyribonucleic acid; DNA; DNA - genetics; DNA - metabolism; DNA-peptide complex; Efficiency; Flowers & plants; Gene expression; Gene transfer; Gene Transfer Techniques; Genes; Genomes; Gold; Membranes; Mitochondria; Mitochondrial DNA; Nanoluc; Particle bombardment; Particle bombardment-assisted peptide-mediated gene transfer (PBPT); Peptides; Peptides - genetics; Permeability; Plant cells; Plant genetics; Plant mitochondria; Plants; Research Note; Seeds; Transformation, Genetic; Japan; United States > US; Nanoluc; Particle bombardment-assisted peptide-mediated gene transfer (PBPT); DNA-peptide complex

A centrifugation-assisted peptide-mediated gene transfer (CAPT) method was recently developed as an efficient system for gene delivery into plant cells. However, the gene transfer efficiency of CAPT into plant cells was not entirely satisfactory for detecting transient expression of a transgene driv...