Particle bombardment-assisted peptide-mediated gene transfer for highly efficient transient assay

A centrifugation-assisted peptide-mediated gene transfer (CAPT) method was recently developed as an efficient system for gene delivery into plant cells. However, the gene transfer efficiency of CAPT into plant cells was not entirely satisfactory for detecting transient expression of a transgene driv...

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Published inBMC research notes Vol. 16; no. 1; p. 46
Main Authors Kimura, Mitsuhiro, Endo, Akira, Nagira, Yozo, Yoshizumi, Takeshi
Format Journal Article
LanguageEnglish
Published England BioMed Central Ltd 06.04.2023
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Abstract A centrifugation-assisted peptide-mediated gene transfer (CAPT) method was recently developed as an efficient system for gene delivery into plant cells. However, the gene transfer efficiency of CAPT into plant cells was not entirely satisfactory for detecting transient expression of a transgene driven into mitochondria. Here, we report a new gene delivery system using a method called particle bombardment-assisted peptide-mediated gene transfer (PBPT). We investigated various parameters of the PBPT method to increase transient gene expression efficiency in Brassica campestris. The optimal conditions for PBPT were a single bombardment with gold particles coated with a DNA‒peptide complex (6 µg of DNA and 2 µg of peptide) at an acceleration pressure of 5 kg/cm and a target distance of 12.5 cm. Moreover, bombardment under the optimal conditions successfully transferred the transgene into the cells of other plant species, namely B. juncea and tomato. Thus, we developed a PBPT method for highly efficient delivery of a DNA‒peptide complex into plant mitochondria.
AbstractList Abstract Objective A centrifugation-assisted peptide-mediated gene transfer (CAPT) method was recently developed as an efficient system for gene delivery into plant cells. However, the gene transfer efficiency of CAPT into plant cells was not entirely satisfactory for detecting transient expression of a transgene driven into mitochondria. Here, we report a new gene delivery system using a method called particle bombardment-assisted peptide-mediated gene transfer (PBPT). Results We investigated various parameters of the PBPT method to increase transient gene expression efficiency in Brassica campestris. The optimal conditions for PBPT were a single bombardment with gold particles coated with a DNA‒peptide complex (6 µg of DNA and 2 µg of peptide) at an acceleration pressure of 5 kg/cm2 and a target distance of 12.5 cm. Moreover, bombardment under the optimal conditions successfully transferred the transgene into the cells of other plant species, namely B. juncea and tomato. Thus, we developed a PBPT method for highly efficient delivery of a DNA‒peptide complex into plant mitochondria.
A centrifugation-assisted peptide-mediated gene transfer (CAPT) method was recently developed as an efficient system for gene delivery into plant cells. However, the gene transfer efficiency of CAPT into plant cells was not entirely satisfactory for detecting transient expression of a transgene driven into mitochondria. Here, we report a new gene delivery system using a method called particle bombardment-assisted peptide-mediated gene transfer (PBPT). We investigated various parameters of the PBPT method to increase transient gene expression efficiency in Brassica campestris. The optimal conditions for PBPT were a single bombardment with gold particles coated with a DNA-peptide complex (6 [micro]g of DNA and 2 [micro]g of peptide) at an acceleration pressure of 5 kg/cm.sup.2 and a target distance of 12.5 cm. Moreover, bombardment under the optimal conditions successfully transferred the transgene into the cells of other plant species, namely B. juncea and tomato. Thus, we developed a PBPT method for highly efficient delivery of a DNA-peptide complex into plant mitochondria.
Objective A centrifugation-assisted peptide-mediated gene transfer (CAPT) method was recently developed as an efficient system for gene delivery into plant cells. However, the gene transfer efficiency of CAPT into plant cells was not entirely satisfactory for detecting transient expression of a transgene driven into mitochondria. Here, we report a new gene delivery system using a method called particle bombardment-assisted peptide-mediated gene transfer (PBPT). Results We investigated various parameters of the PBPT method to increase transient gene expression efficiency in Brassica campestris. The optimal conditions for PBPT were a single bombardment with gold particles coated with a DNA-peptide complex (6 [micro]g of DNA and 2 [micro]g of peptide) at an acceleration pressure of 5 kg/cm.sup.2 and a target distance of 12.5 cm. Moreover, bombardment under the optimal conditions successfully transferred the transgene into the cells of other plant species, namely B. juncea and tomato. Thus, we developed a PBPT method for highly efficient delivery of a DNA-peptide complex into plant mitochondria. Keywords: Particle bombardment-assisted peptide-mediated gene transfer (PBPT), DNA-peptide complex, Nanoluc
OBJECTIVEA centrifugation-assisted peptide-mediated gene transfer (CAPT) method was recently developed as an efficient system for gene delivery into plant cells. However, the gene transfer efficiency of CAPT into plant cells was not entirely satisfactory for detecting transient expression of a transgene driven into mitochondria. Here, we report a new gene delivery system using a method called particle bombardment-assisted peptide-mediated gene transfer (PBPT). RESULTSWe investigated various parameters of the PBPT method to increase transient gene expression efficiency in Brassica campestris. The optimal conditions for PBPT were a single bombardment with gold particles coated with a DNA‒peptide complex (6 µg of DNA and 2 µg of peptide) at an acceleration pressure of 5 kg/cm2 and a target distance of 12.5 cm. Moreover, bombardment under the optimal conditions successfully transferred the transgene into the cells of other plant species, namely B. juncea and tomato. Thus, we developed a PBPT method for highly efficient delivery of a DNA‒peptide complex into plant mitochondria.
A centrifugation-assisted peptide-mediated gene transfer (CAPT) method was recently developed as an efficient system for gene delivery into plant cells. However, the gene transfer efficiency of CAPT into plant cells was not entirely satisfactory for detecting transient expression of a transgene driven into mitochondria. Here, we report a new gene delivery system using a method called particle bombardment-assisted peptide-mediated gene transfer (PBPT). We investigated various parameters of the PBPT method to increase transient gene expression efficiency in Brassica campestris. The optimal conditions for PBPT were a single bombardment with gold particles coated with a DNA‒peptide complex (6 µg of DNA and 2 µg of peptide) at an acceleration pressure of 5 kg/cm and a target distance of 12.5 cm. Moreover, bombardment under the optimal conditions successfully transferred the transgene into the cells of other plant species, namely B. juncea and tomato. Thus, we developed a PBPT method for highly efficient delivery of a DNA‒peptide complex into plant mitochondria.
Abstract Objective A centrifugation-assisted peptide-mediated gene transfer (CAPT) method was recently developed as an efficient system for gene delivery into plant cells. However, the gene transfer efficiency of CAPT into plant cells was not entirely satisfactory for detecting transient expression of a transgene driven into mitochondria. Here, we report a new gene delivery system using a method called particle bombardment-assisted peptide-mediated gene transfer (PBPT). Results We investigated various parameters of the PBPT method to increase transient gene expression efficiency in Brassica campestris . The optimal conditions for PBPT were a single bombardment with gold particles coated with a DNA‒peptide complex (6 µg of DNA and 2 µg of peptide) at an acceleration pressure of 5 kg/cm 2 and a target distance of 12.5 cm. Moreover, bombardment under the optimal conditions successfully transferred the transgene into the cells of other plant species, namely B. juncea and tomato. Thus, we developed a PBPT method for highly efficient delivery of a DNA‒peptide complex into plant mitochondria.
ArticleNumber 46
Audience Academic
Author Nagira, Yozo
Endo, Akira
Yoshizumi, Takeshi
Kimura, Mitsuhiro
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  organization: Faculty of Agriculture, Kyushu University, Fukuoka, Japan. mitsukimura@agr.kyushu-u.ac.jp
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  givenname: Takeshi
  surname: Yoshizumi
  fullname: Yoshizumi, Takeshi
  email: yoshidumi@takasaki-u.ac.jp
  organization: Faculty of Agriculture, Takasaki University of Health and Welfare, Takasaki-shi, Gunma, Japan. yoshidumi@takasaki-u.ac.jp
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Cites_doi 10.1007/BF02824002
10.1021/acs.bioconjchem.6b00112
10.1021/bm301275g
10.1111/tpj.15495
10.1038/srep07751
10.1016/j.tplants.2017.10.001
10.5511/plantbiotechnology.18.1115a
10.1038/nprot.2006.145
10.1186/s13007-017-0257-4
10.3389/fpls.2017.02221
10.3390/ijms21144854
10.1038/bmt.2012.244
10.1080/15592324.2018.1441657
10.5511/plantbiotechnology.22.415
10.1021/acs.biomac.8b00323
10.5511/plantbiotechnology.15.217
10.1016/j.xplc.2021.100141
10.5511/plantbiotechnology.14.1210b
10.1073/pnas.081065198
10.1104/pp.111.186957
10.1111/j.1399-3054.1962.tb08052.x
10.1038/s41598-018-36466-1
10.5511/plantbiotechnology.20.0503a
10.1007/978-1-0716-0356-7_6
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Issue 1
Keywords Nanoluc
Particle bombardment-assisted peptide-mediated gene transfer (PBPT)
DNA-peptide complex
Language English
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References S Arimura (6320_CR8) 2005; 22
K Midorikawa (6320_CR11) 2019; 9
Y Kanda (6320_CR18) 2013; 48
T Yoshizumi (6320_CR6) 2018; 19
B Lacroix (6320_CR27) 2020; 2124
J-A Chuah (6320_CR5) 2015; 5
M Crumpton-Taylor (6320_CR9) 2012; 158
MW Hameed (6320_CR13) 2017; 13
IM Møller (6320_CR2) 2021; 108
CG England (6320_CR12) 2016; 27
M Mookkan (6320_CR21) 2018; 13
E Helenius (6320_CR25) 2000; 18
T Murashige (6320_CR10) 1962; 15
YY Yamamoto (6320_CR17) 1998; 15
YJ Kim (6320_CR3) 2018; 23
AM van der Bliek (6320_CR1) 2017; 207
Y Yu (6320_CR26) 2020; 21
DJ Stephens (6320_CR19) 2001; 98
A Okuzaki (6320_CR28) 2020; 37
D Daniels (6320_CR22) 2018; 4
S Dhir (6320_CR24) 2010; 9
JA O’Brien (6320_CR20) 2006; 1
S Li (6320_CR4) 2021; 2
M Kimura (6320_CR7) 2019; 36
M Lakshmanan (6320_CR15) 2015; 32
R Akashi (6320_CR16) 2002; 47
Y Zhao (6320_CR23) 2018; 8
M Lakshmanan (6320_CR14) 2013; 14
References_xml – volume: 18
  start-page: 287
  issue: 3
  year: 2000
  ident: 6320_CR25
  publication-title: Plant Mol Biol Report
  doi: 10.1007/BF02824002
  contributor:
    fullname: E Helenius
– volume: 27
  start-page: 1175
  issue: 5
  year: 2016
  ident: 6320_CR12
  publication-title: Bioconjug chem
  doi: 10.1021/acs.bioconjchem.6b00112
  contributor:
    fullname: CG England
– volume: 14
  start-page: 10
  issue: 1
  year: 2013
  ident: 6320_CR14
  publication-title: Biomacromolecules
  doi: 10.1021/bm301275g
  contributor:
    fullname: M Lakshmanan
– volume: 47
  start-page: 588
  issue: 6
  year: 2002
  ident: 6320_CR16
  publication-title: Grassl Sci
  contributor:
    fullname: R Akashi
– volume: 108
  start-page: 912
  issue: 4
  year: 2021
  ident: 6320_CR2
  publication-title: Plant J
  doi: 10.1111/tpj.15495
  contributor:
    fullname: IM Møller
– volume: 5
  start-page: 7751
  issue: 1
  year: 2015
  ident: 6320_CR5
  publication-title: Sci Rep
  doi: 10.1038/srep07751
  contributor:
    fullname: J-A Chuah
– volume: 23
  start-page: 53
  issue: 1
  year: 2018
  ident: 6320_CR3
  publication-title: Trends Plant Sci
  doi: 10.1016/j.tplants.2017.10.001
  contributor:
    fullname: YJ Kim
– volume: 36
  start-page: 49
  issue: 1
  year: 2019
  ident: 6320_CR7
  publication-title: Plant Biotechnol
  doi: 10.5511/plantbiotechnology.18.1115a
  contributor:
    fullname: M Kimura
– volume: 9
  start-page: 6460
  issue: 39
  year: 2010
  ident: 6320_CR24
  publication-title: Afr J Biotechnol
  contributor:
    fullname: S Dhir
– volume: 1
  start-page: 977
  issue: 2
  year: 2006
  ident: 6320_CR20
  publication-title: Nat protocols
  doi: 10.1038/nprot.2006.145
  contributor:
    fullname: JA O’Brien
– volume: 207
  start-page: 843
  issue: 3
  year: 2017
  ident: 6320_CR1
  publication-title: Cell Biology of the Mitochondrion Genetics
  contributor:
    fullname: AM van der Bliek
– volume: 13
  start-page: 112
  issue: 1
  year: 2017
  ident: 6320_CR13
  publication-title: Plant Methods
  doi: 10.1186/s13007-017-0257-4
  contributor:
    fullname: MW Hameed
– volume: 4
  start-page: 42
  issue: 9
  year: 2018
  ident: 6320_CR22
  publication-title: Int J Adv Sci Res Eng
  contributor:
    fullname: D Daniels
– volume: 8
  start-page: 2221
  year: 2018
  ident: 6320_CR23
  publication-title: Front Plant Sci
  doi: 10.3389/fpls.2017.02221
  contributor:
    fullname: Y Zhao
– volume: 21
  start-page: 1
  issue: 14
  year: 2020
  ident: 6320_CR26
  publication-title: Int J Mol Sci
  doi: 10.3390/ijms21144854
  contributor:
    fullname: Y Yu
– volume: 48
  start-page: 452
  issue: 3
  year: 2013
  ident: 6320_CR18
  publication-title: Bone Marrow Transplantat
  doi: 10.1038/bmt.2012.244
  contributor:
    fullname: Y Kanda
– volume: 13
  start-page: e1441657
  issue: 4
  year: 2018
  ident: 6320_CR21
  publication-title: Plant Signal Behav
  doi: 10.1080/15592324.2018.1441657
  contributor:
    fullname: M Mookkan
– volume: 22
  start-page: 415
  issue: 5
  year: 2005
  ident: 6320_CR8
  publication-title: Plant Biotechnol
  doi: 10.5511/plantbiotechnology.22.415
  contributor:
    fullname: S Arimura
– volume: 19
  start-page: 1582
  issue: 5
  year: 2018
  ident: 6320_CR6
  publication-title: Biomacromolecules
  doi: 10.1021/acs.biomac.8b00323
  contributor:
    fullname: T Yoshizumi
– volume: 15
  start-page: 217
  issue: 4
  year: 1998
  ident: 6320_CR17
  publication-title: Plant Biotechnol
  doi: 10.5511/plantbiotechnology.15.217
  contributor:
    fullname: YY Yamamoto
– volume: 2
  start-page: 100141
  issue: 2
  year: 2021
  ident: 6320_CR4
  publication-title: Plant Commun
  doi: 10.1016/j.xplc.2021.100141
  contributor:
    fullname: S Li
– volume: 32
  start-page: 39
  year: 2015
  ident: 6320_CR15
  publication-title: Plant Biotechnol
  doi: 10.5511/plantbiotechnology.14.1210b
  contributor:
    fullname: M Lakshmanan
– volume: 98
  start-page: 4295
  issue: 8
  year: 2001
  ident: 6320_CR19
  publication-title: Proc Natl Acad Sci U S A
  doi: 10.1073/pnas.081065198
  contributor:
    fullname: DJ Stephens
– volume: 158
  start-page: 905
  issue: 2
  year: 2012
  ident: 6320_CR9
  publication-title: Plant Physiol
  doi: 10.1104/pp.111.186957
  contributor:
    fullname: M Crumpton-Taylor
– volume: 15
  start-page: 473
  issue: 3
  year: 1962
  ident: 6320_CR10
  publication-title: Physiol Plant
  doi: 10.1111/j.1399-3054.1962.tb08052.x
  contributor:
    fullname: T Murashige
– volume: 9
  start-page: 1
  issue: 1
  year: 2019
  ident: 6320_CR11
  publication-title: Sci Rep
  doi: 10.1038/s41598-018-36466-1
  contributor:
    fullname: K Midorikawa
– volume: 37
  start-page: 223
  issue: 2
  year: 2020
  ident: 6320_CR28
  publication-title: Plant Biotechnol
  doi: 10.5511/plantbiotechnology.20.0503a
  contributor:
    fullname: A Okuzaki
– volume: 2124
  start-page: 125
  year: 2020
  ident: 6320_CR27
  publication-title: Methods Mol Biol
  doi: 10.1007/978-1-0716-0356-7_6
  contributor:
    fullname: B Lacroix
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Snippet A centrifugation-assisted peptide-mediated gene transfer (CAPT) method was recently developed as an efficient system for gene delivery into plant cells....
Abstract Objective A centrifugation-assisted peptide-mediated gene transfer (CAPT) method was recently developed as an efficient system for gene delivery into...
Objective A centrifugation-assisted peptide-mediated gene transfer (CAPT) method was recently developed as an efficient system for gene delivery into plant...
ObjectiveA centrifugation-assisted peptide-mediated gene transfer (CAPT) method was recently developed as an efficient system for gene delivery into plant...
OBJECTIVEA centrifugation-assisted peptide-mediated gene transfer (CAPT) method was recently developed as an efficient system for gene delivery into plant...
Abstract Objective A centrifugation-assisted peptide-mediated gene transfer (CAPT) method was recently developed as an efficient system for gene delivery into...
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StartPage 46
SubjectTerms Centrifugation
Chloroplasts
Cytotoxicity
Deoxyribonucleic acid
DNA
DNA - genetics
DNA - metabolism
DNA-peptide complex
Efficiency
Flowers & plants
Gene expression
Gene transfer
Gene Transfer Techniques
Genes
Genomes
Gold
Membranes
Mitochondria
Mitochondrial DNA
Nanoluc
Particle bombardment
Particle bombardment-assisted peptide-mediated gene transfer (PBPT)
Peptides
Peptides - genetics
Permeability
Plant cells
Plant genetics
Plant mitochondria
Plants
Research Note
Seeds
Transformation, Genetic
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Title Particle bombardment-assisted peptide-mediated gene transfer for highly efficient transient assay
URI https://www.ncbi.nlm.nih.gov/pubmed/37024962
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Volume 16
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