Particle bombardment-assisted peptide-mediated gene transfer for highly efficient transient assay

A centrifugation-assisted peptide-mediated gene transfer (CAPT) method was recently developed as an efficient system for gene delivery into plant cells. However, the gene transfer efficiency of CAPT into plant cells was not entirely satisfactory for detecting transient expression of a transgene driv...

Full description

Saved in:
Bibliographic Details
Published inBMC research notes Vol. 16; no. 1; p. 46
Main Authors Kimura, Mitsuhiro, Endo, Akira, Nagira, Yozo, Yoshizumi, Takeshi
Format Journal Article
LanguageEnglish
Published England BioMed Central Ltd 06.04.2023
BioMed Central
BMC
Subjects
Centrifugation
Chloroplasts
Cytotoxicity
Deoxyribonucleic acid
DNA
DNA - genetics
DNA - metabolism
DNA-peptide complex
Efficiency
Flowers & plants
Gene expression
Gene transfer
Gene Transfer Techniques
Genes
Genomes
Gold
Membranes
Mitochondria
Mitochondrial DNA
Nanoluc
Particle bombardment
Particle bombardment-assisted peptide-mediated gene transfer (PBPT)
Peptides
Peptides - genetics
Permeability
Plant cells
Plant genetics
Plant mitochondria
Plants
Research Note
Seeds
Transformation, Genetic
Japan
United States > US
Nanoluc
Particle bombardment-assisted peptide-mediated gene transfer (PBPT)
DNA-peptide complex
Online AccessGet full text

Cover

Abstract A centrifugation-assisted peptide-mediated gene transfer (CAPT) method was recently developed as an efficient system for gene delivery into plant cells. However, the gene transfer efficiency of CAPT into plant cells was not entirely satisfactory for detecting transient expression of a transgene driven into mitochondria. Here, we report a new gene delivery system using a method called particle bombardment-assisted peptide-mediated gene transfer (PBPT). We investigated various parameters of the PBPT method to increase transient gene expression efficiency in Brassica campestris. The optimal conditions for PBPT were a single bombardment with gold particles coated with a DNA‒peptide complex (6 µg of DNA and 2 µg of peptide) at an acceleration pressure of 5 kg/cm and a target distance of 12.5 cm. Moreover, bombardment under the optimal conditions successfully transferred the transgene into the cells of other plant species, namely B. juncea and tomato. Thus, we developed a PBPT method for highly efficient delivery of a DNA‒peptide complex into plant mitochondria.
AbstractList Abstract Objective A centrifugation-assisted peptide-mediated gene transfer (CAPT) method was recently developed as an efficient system for gene delivery into plant cells. However, the gene transfer efficiency of CAPT into plant cells was not entirely satisfactory for detecting transient expression of a transgene driven into mitochondria. Here, we report a new gene delivery system using a method called particle bombardment-assisted peptide-mediated gene transfer (PBPT). Results We investigated various parameters of the PBPT method to increase transient gene expression efficiency in Brassica campestris. The optimal conditions for PBPT were a single bombardment with gold particles coated with a DNA‒peptide complex (6 µg of DNA and 2 µg of peptide) at an acceleration pressure of 5 kg/cm2 and a target distance of 12.5 cm. Moreover, bombardment under the optimal conditions successfully transferred the transgene into the cells of other plant species, namely B. juncea and tomato. Thus, we developed a PBPT method for highly efficient delivery of a DNA‒peptide complex into plant mitochondria.
A centrifugation-assisted peptide-mediated gene transfer (CAPT) method was recently developed as an efficient system for gene delivery into plant cells. However, the gene transfer efficiency of CAPT into plant cells was not entirely satisfactory for detecting transient expression of a transgene driven into mitochondria. Here, we report a new gene delivery system using a method called particle bombardment-assisted peptide-mediated gene transfer (PBPT). We investigated various parameters of the PBPT method to increase transient gene expression efficiency in Brassica campestris. The optimal conditions for PBPT were a single bombardment with gold particles coated with a DNA-peptide complex (6 [micro]g of DNA and 2 [micro]g of peptide) at an acceleration pressure of 5 kg/cm.sup.2 and a target distance of 12.5 cm. Moreover, bombardment under the optimal conditions successfully transferred the transgene into the cells of other plant species, namely B. juncea and tomato. Thus, we developed a PBPT method for highly efficient delivery of a DNA-peptide complex into plant mitochondria.
Objective A centrifugation-assisted peptide-mediated gene transfer (CAPT) method was recently developed as an efficient system for gene delivery into plant cells. However, the gene transfer efficiency of CAPT into plant cells was not entirely satisfactory for detecting transient expression of a transgene driven into mitochondria. Here, we report a new gene delivery system using a method called particle bombardment-assisted peptide-mediated gene transfer (PBPT). Results We investigated various parameters of the PBPT method to increase transient gene expression efficiency in Brassica campestris. The optimal conditions for PBPT were a single bombardment with gold particles coated with a DNA-peptide complex (6 [micro]g of DNA and 2 [micro]g of peptide) at an acceleration pressure of 5 kg/cm.sup.2 and a target distance of 12.5 cm. Moreover, bombardment under the optimal conditions successfully transferred the transgene into the cells of other plant species, namely B. juncea and tomato. Thus, we developed a PBPT method for highly efficient delivery of a DNA-peptide complex into plant mitochondria. Keywords: Particle bombardment-assisted peptide-mediated gene transfer (PBPT), DNA-peptide complex, Nanoluc
OBJECTIVEA centrifugation-assisted peptide-mediated gene transfer (CAPT) method was recently developed as an efficient system for gene delivery into plant cells. However, the gene transfer efficiency of CAPT into plant cells was not entirely satisfactory for detecting transient expression of a transgene driven into mitochondria. Here, we report a new gene delivery system using a method called particle bombardment-assisted peptide-mediated gene transfer (PBPT). RESULTSWe investigated various parameters of the PBPT method to increase transient gene expression efficiency in Brassica campestris. The optimal conditions for PBPT were a single bombardment with gold particles coated with a DNA‒peptide complex (6 µg of DNA and 2 µg of peptide) at an acceleration pressure of 5 kg/cm2 and a target distance of 12.5 cm. Moreover, bombardment under the optimal conditions successfully transferred the transgene into the cells of other plant species, namely B. juncea and tomato. Thus, we developed a PBPT method for highly efficient delivery of a DNA‒peptide complex into plant mitochondria.
A centrifugation-assisted peptide-mediated gene transfer (CAPT) method was recently developed as an efficient system for gene delivery into plant cells. However, the gene transfer efficiency of CAPT into plant cells was not entirely satisfactory for detecting transient expression of a transgene driven into mitochondria. Here, we report a new gene delivery system using a method called particle bombardment-assisted peptide-mediated gene transfer (PBPT). We investigated various parameters of the PBPT method to increase transient gene expression efficiency in Brassica campestris. The optimal conditions for PBPT were a single bombardment with gold particles coated with a DNA‒peptide complex (6 µg of DNA and 2 µg of peptide) at an acceleration pressure of 5 kg/cm and a target distance of 12.5 cm. Moreover, bombardment under the optimal conditions successfully transferred the transgene into the cells of other plant species, namely B. juncea and tomato. Thus, we developed a PBPT method for highly efficient delivery of a DNA‒peptide complex into plant mitochondria.
Abstract Objective A centrifugation-assisted peptide-mediated gene transfer (CAPT) method was recently developed as an efficient system for gene delivery into plant cells. However, the gene transfer efficiency of CAPT into plant cells was not entirely satisfactory for detecting transient expression of a transgene driven into mitochondria. Here, we report a new gene delivery system using a method called particle bombardment-assisted peptide-mediated gene transfer (PBPT). Results We investigated various parameters of the PBPT method to increase transient gene expression efficiency in Brassica campestris . The optimal conditions for PBPT were a single bombardment with gold particles coated with a DNA‒peptide complex (6 µg of DNA and 2 µg of peptide) at an acceleration pressure of 5 kg/cm 2 and a target distance of 12.5 cm. Moreover, bombardment under the optimal conditions successfully transferred the transgene into the cells of other plant species, namely B. juncea and tomato. Thus, we developed a PBPT method for highly efficient delivery of a DNA‒peptide complex into plant mitochondria.
ArticleNumber 46
Audience Academic
Author Nagira, Yozo
Endo, Akira
Yoshizumi, Takeshi
Kimura, Mitsuhiro
Author_xml – sequence: 1
  givenname: Mitsuhiro
  surname: Kimura
  fullname: Kimura, Mitsuhiro
  email: mitsukimura@agr.kyushu-u.ac.jp, mitsukimura@agr.kyushu-u.ac.jp
  organization: Faculty of Agriculture, Kyushu University, Fukuoka, Japan. mitsukimura@agr.kyushu-u.ac.jp
– sequence: 2
  givenname: Akira
  surname: Endo
  fullname: Endo, Akira
  organization: Agri-Bio Research Center, Agri-Bio & Supplement Research Laboratories, Kaneka Corporation, Iwata, Shizuoka, Japan
– sequence: 3
  givenname: Yozo
  surname: Nagira
  fullname: Nagira, Yozo
  organization: Agri-Bio Research Center, Agri-Bio & Supplement Research Laboratories, Kaneka Corporation, Iwata, Shizuoka, Japan
– sequence: 4
  givenname: Takeshi
  surname: Yoshizumi
  fullname: Yoshizumi, Takeshi
  email: yoshidumi@takasaki-u.ac.jp
  organization: Faculty of Agriculture, Takasaki University of Health and Welfare, Takasaki-shi, Gunma, Japan. yoshidumi@takasaki-u.ac.jp
BackLink https://www.ncbi.nlm.nih.gov/pubmed/37024962$$D View this record in MEDLINE/PubMed
BookMark eNptkktv1DAUhS1URNuBP8ACRWIDi5TrRxxnhaqKx0iVygLYWrZzk3GVxIOdQcy_x_OgdBDKItb1OZ91rs4lOZvChIS8pHBFqZLvEuUURAmMlyA5g5I_IRe0rmQJFcDZo_M5uUzpHkBSpegzcs5rYKKR7IKYLybO3g1Y2DBaE9sRp7k0Kfk0Y1uscT37FssRW292gx4nLOZoptRhLLoQi5XvV8O2wK7zzmfz4XZ_yhizfU6edmZI-OL4X5BvHz98vflc3t59Wt5c35ZO0mouFQJaZmXmULBNw1jTUtsYLqwwwgIFpIpyC8xayZBZB05RwRpjneOm5QuyPHDbYO71OvrRxK0Oxuv9IMReH6NqwWuJaJisFAhZgW0p55Z1ClUjaIOZ9f7AWm9sju5ymGiGE-jpzeRXug8_NQVQoLjMhDdHQgw_NphmPfrkcBjMhGGTNKsbVUNOWWXp63-k92ETp7wrzRRwYFBX9V9Vb3ICP3UhP-x2UH1dCyGh2j28IFf_UeWvxdG7XJ_O5_mJ4e2JIWtm_DX3ZpOSXt59P9Wyg9bFkFLE7mEhFPSukfrQSJ0bqfeN1DybXj1e5YPlTwX5b1Us3HI
Cites_doi 10.1007/BF02824002
10.1021/acs.bioconjchem.6b00112
10.1021/bm301275g
10.1111/tpj.15495
10.1038/srep07751
10.1016/j.tplants.2017.10.001
10.5511/plantbiotechnology.18.1115a
10.1038/nprot.2006.145
10.1186/s13007-017-0257-4
10.3389/fpls.2017.02221
10.3390/ijms21144854
10.1038/bmt.2012.244
10.1080/15592324.2018.1441657
10.5511/plantbiotechnology.22.415
10.1021/acs.biomac.8b00323
10.5511/plantbiotechnology.15.217
10.1016/j.xplc.2021.100141
10.5511/plantbiotechnology.14.1210b
10.1073/pnas.081065198
10.1104/pp.111.186957
10.1111/j.1399-3054.1962.tb08052.x
10.1038/s41598-018-36466-1
10.5511/plantbiotechnology.20.0503a
10.1007/978-1-0716-0356-7_6
ContentType Journal Article
Copyright 2023. The Author(s).
COPYRIGHT 2023 BioMed Central Ltd.
2023. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
The Author(s) 2023
Copyright_xml – notice: 2023. The Author(s).
– notice: COPYRIGHT 2023 BioMed Central Ltd.
– notice: 2023. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
– notice: The Author(s) 2023
DBID CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
IOV
3V.
7X7
7XB
88E
8FE
8FH
8FI
8FJ
8FK
ABUWG
AFKRA
AZQEC
BBNVY
BENPR
BHPHI
CCPQU
DWQXO
FYUFA
GHDGH
GNUQQ
HCIFZ
K9.
LK8
M0S
M1P
M7P
PIMPY
PQEST
PQQKQ
PQUKI
PRINS
7X8
5PM
DOA
DOI 10.1186/s13104-023-06320-3
DatabaseName Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
CrossRef
Opposing Viewpoints In Context
ProQuest Central (Corporate)
Health & Medical Collection
ProQuest Central (purchase pre-March 2016)
Medical Database (Alumni Edition)
ProQuest SciTech Collection
ProQuest Natural Science Collection
Hospital Premium Collection
Hospital Premium Collection (Alumni Edition)
ProQuest Central (Alumni) (purchase pre-March 2016)
ProQuest Central (Alumni)
ProQuest Central
ProQuest Central Essentials
Biological Science Collection
ProQuest Central
Natural Science Collection
ProQuest One Community College
ProQuest Central Korea
Health Research Premium Collection
Health Research Premium Collection (Alumni)
ProQuest Central Student
SciTech Premium Collection
ProQuest Health & Medical Complete (Alumni)
Biological Sciences
Health & Medical Collection (Alumni Edition)
PML(ProQuest Medical Library)
Biological Science Database
Publicly Available Content Database
ProQuest One Academic Eastern Edition (DO NOT USE)
ProQuest One Academic
ProQuest One Academic UKI Edition
ProQuest Central China
MEDLINE - Academic
PubMed Central (Full Participant titles)
Directory of Open Access Journals
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
CrossRef
Publicly Available Content Database
ProQuest Central Student
ProQuest Central Essentials
ProQuest Health & Medical Complete (Alumni)
ProQuest Central (Alumni Edition)
SciTech Premium Collection
ProQuest One Community College
ProQuest Natural Science Collection
ProQuest Central China
ProQuest Central
Health Research Premium Collection
Health and Medicine Complete (Alumni Edition)
Natural Science Collection
ProQuest Central Korea
Biological Science Collection
ProQuest Medical Library (Alumni)
ProQuest Biological Science Collection
ProQuest One Academic Eastern Edition
ProQuest Hospital Collection
Health Research Premium Collection (Alumni)
Biological Science Database
ProQuest SciTech Collection
ProQuest Hospital Collection (Alumni)
ProQuest Health & Medical Complete
ProQuest Medical Library
ProQuest One Academic UKI Edition
ProQuest One Academic
ProQuest Central (Alumni)
MEDLINE - Academic
DatabaseTitleList


MEDLINE - Academic

MEDLINE
Publicly Available Content Database
CrossRef
Database_xml – sequence: 1
  dbid: DOA
  name: DOAJ Directory of Open Access Journals
  url: https://www.doaj.org/
  sourceTypes: Open Website
– sequence: 2
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 3
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
– sequence: 4
  dbid: BENPR
  name: ProQuest Central
  url: https://www.proquest.com/central
  sourceTypes: Aggregation Database
DeliveryMethod fulltext_linktorsrc
Discipline Biology
EISSN 1756-0500
EndPage 46
ExternalDocumentID oai_doaj_org_article_4376eea265804650bd133b2f8e89419e
A744605808
10_1186_s13104_023_06320_3
37024962
Genre Journal Article
GeographicLocations Japan
United States--US
GeographicLocations_xml – name: Japan
– name: United States--US
GrantInformation_xml – fundername: Japan Society for the Promotion of Science,Japan
  grantid: 20K21319
– fundername: Japan Society for the Promotion of Science
  grantid: 18K05638
– fundername: ;
  grantid: 18K05638
– fundername: ;
– fundername: ;
  grantid: 20K21319
GroupedDBID ---
-A0
0R~
23N
2WC
3V.
53G
5GY
5VS
6J9
7X7
88E
8FE
8FH
8FI
8FJ
AAFWJ
AAJSJ
ABDBF
ABUWG
ACGFO
ACGFS
ACIHN
ACMJI
ACPRK
ACRMQ
ADBBV
ADINQ
ADRAZ
ADUKV
AEAQA
AFKRA
AFPKN
AHBYD
AHMBA
AHYZX
ALIPV
ALMA_UNASSIGNED_HOLDINGS
AMKLP
AMTXH
AOIJS
BAPOH
BAWUL
BBNVY
BCNDV
BENPR
BFQNJ
BHPHI
BMC
BPHCQ
BVXVI
C24
C6C
CCPQU
CGR
CS3
CUY
CVF
DIK
E3Z
EBD
EBLON
EBS
ECM
EIF
EMOBN
ESX
F5P
FYUFA
GROUPED_DOAJ
GX1
HCIFZ
HMCUK
HYE
IAO
IEA
IHR
INH
INR
IOV
ITC
KQ8
LK8
M1P
M48
M7P
MK0
M~E
NPM
O5R
O5S
OK1
P2P
PGMZT
PIMPY
PQQKQ
PROAC
PSQYO
RBZ
RNS
ROL
RPM
RSV
SBL
SOJ
SV3
TR2
TUS
UKHRP
~8M
AAYXX
CITATION
ABVAZ
AFGXO
AFNRJ
7XB
8FK
AZQEC
DWQXO
GNUQQ
K9.
PQEST
PQUKI
PRINS
7X8
5PM
ID FETCH-LOGICAL-c615t-8e0eb2b6eff10b99229d1b9a34b4a4b010e1813b02bb62e2bc0c81429abcc3ad3
IEDL.DBID RPM
ISSN 1756-0500
IngestDate Tue Oct 22 15:11:14 EDT 2024
Tue Sep 17 21:33:32 EDT 2024
Sat Oct 05 05:18:08 EDT 2024
Thu Oct 10 22:10:03 EDT 2024
Thu Feb 22 23:40:15 EST 2024
Fri Feb 02 04:17:45 EST 2024
Sat Sep 28 21:30:15 EDT 2024
Thu Sep 12 19:38:15 EDT 2024
Sat Sep 28 08:16:59 EDT 2024
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 1
Keywords Nanoluc
Particle bombardment-assisted peptide-mediated gene transfer (PBPT)
DNA-peptide complex
Language English
License 2023. The Author(s).
Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c615t-8e0eb2b6eff10b99229d1b9a34b4a4b010e1813b02bb62e2bc0c81429abcc3ad3
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
OpenAccessLink https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10080836/
PMID 37024962
PQID 2803020757
PQPubID 55247
PageCount 1
ParticipantIDs doaj_primary_oai_doaj_org_article_4376eea265804650bd133b2f8e89419e
pubmedcentral_primary_oai_pubmedcentral_nih_gov_10080836
proquest_miscellaneous_2798709225
proquest_journals_2803020757
gale_infotracmisc_A744605808
gale_infotracacademiconefile_A744605808
gale_incontextgauss_IOV_A744605808
crossref_primary_10_1186_s13104_023_06320_3
pubmed_primary_37024962
PublicationCentury 2000
PublicationDate 2023-04-06
PublicationDateYYYYMMDD 2023-04-06
PublicationDate_xml – month: 04
  year: 2023
  text: 2023-04-06
  day: 06
PublicationDecade 2020
PublicationPlace England
PublicationPlace_xml – name: England
– name: London
PublicationTitle BMC research notes
PublicationTitleAlternate BMC Res Notes
PublicationYear 2023
Publisher BioMed Central Ltd
BioMed Central
BMC
Publisher_xml – name: BioMed Central Ltd
– name: BioMed Central
– name: BMC
References S Arimura (6320_CR8) 2005; 22
K Midorikawa (6320_CR11) 2019; 9
Y Kanda (6320_CR18) 2013; 48
T Yoshizumi (6320_CR6) 2018; 19
B Lacroix (6320_CR27) 2020; 2124
J-A Chuah (6320_CR5) 2015; 5
M Crumpton-Taylor (6320_CR9) 2012; 158
MW Hameed (6320_CR13) 2017; 13
IM Møller (6320_CR2) 2021; 108
CG England (6320_CR12) 2016; 27
M Mookkan (6320_CR21) 2018; 13
E Helenius (6320_CR25) 2000; 18
T Murashige (6320_CR10) 1962; 15
YY Yamamoto (6320_CR17) 1998; 15
YJ Kim (6320_CR3) 2018; 23
AM van der Bliek (6320_CR1) 2017; 207
Y Yu (6320_CR26) 2020; 21
DJ Stephens (6320_CR19) 2001; 98
A Okuzaki (6320_CR28) 2020; 37
D Daniels (6320_CR22) 2018; 4
S Dhir (6320_CR24) 2010; 9
JA O’Brien (6320_CR20) 2006; 1
S Li (6320_CR4) 2021; 2
M Kimura (6320_CR7) 2019; 36
M Lakshmanan (6320_CR15) 2015; 32
R Akashi (6320_CR16) 2002; 47
Y Zhao (6320_CR23) 2018; 8
M Lakshmanan (6320_CR14) 2013; 14
References_xml – volume: 18
  start-page: 287
  issue: 3
  year: 2000
  ident: 6320_CR25
  publication-title: Plant Mol Biol Report
  doi: 10.1007/BF02824002
  contributor:
    fullname: E Helenius
– volume: 27
  start-page: 1175
  issue: 5
  year: 2016
  ident: 6320_CR12
  publication-title: Bioconjug chem
  doi: 10.1021/acs.bioconjchem.6b00112
  contributor:
    fullname: CG England
– volume: 14
  start-page: 10
  issue: 1
  year: 2013
  ident: 6320_CR14
  publication-title: Biomacromolecules
  doi: 10.1021/bm301275g
  contributor:
    fullname: M Lakshmanan
– volume: 47
  start-page: 588
  issue: 6
  year: 2002
  ident: 6320_CR16
  publication-title: Grassl Sci
  contributor:
    fullname: R Akashi
– volume: 108
  start-page: 912
  issue: 4
  year: 2021
  ident: 6320_CR2
  publication-title: Plant J
  doi: 10.1111/tpj.15495
  contributor:
    fullname: IM Møller
– volume: 5
  start-page: 7751
  issue: 1
  year: 2015
  ident: 6320_CR5
  publication-title: Sci Rep
  doi: 10.1038/srep07751
  contributor:
    fullname: J-A Chuah
– volume: 23
  start-page: 53
  issue: 1
  year: 2018
  ident: 6320_CR3
  publication-title: Trends Plant Sci
  doi: 10.1016/j.tplants.2017.10.001
  contributor:
    fullname: YJ Kim
– volume: 36
  start-page: 49
  issue: 1
  year: 2019
  ident: 6320_CR7
  publication-title: Plant Biotechnol
  doi: 10.5511/plantbiotechnology.18.1115a
  contributor:
    fullname: M Kimura
– volume: 9
  start-page: 6460
  issue: 39
  year: 2010
  ident: 6320_CR24
  publication-title: Afr J Biotechnol
  contributor:
    fullname: S Dhir
– volume: 1
  start-page: 977
  issue: 2
  year: 2006
  ident: 6320_CR20
  publication-title: Nat protocols
  doi: 10.1038/nprot.2006.145
  contributor:
    fullname: JA O’Brien
– volume: 207
  start-page: 843
  issue: 3
  year: 2017
  ident: 6320_CR1
  publication-title: Cell Biology of the Mitochondrion Genetics
  contributor:
    fullname: AM van der Bliek
– volume: 13
  start-page: 112
  issue: 1
  year: 2017
  ident: 6320_CR13
  publication-title: Plant Methods
  doi: 10.1186/s13007-017-0257-4
  contributor:
    fullname: MW Hameed
– volume: 4
  start-page: 42
  issue: 9
  year: 2018
  ident: 6320_CR22
  publication-title: Int J Adv Sci Res Eng
  contributor:
    fullname: D Daniels
– volume: 8
  start-page: 2221
  year: 2018
  ident: 6320_CR23
  publication-title: Front Plant Sci
  doi: 10.3389/fpls.2017.02221
  contributor:
    fullname: Y Zhao
– volume: 21
  start-page: 1
  issue: 14
  year: 2020
  ident: 6320_CR26
  publication-title: Int J Mol Sci
  doi: 10.3390/ijms21144854
  contributor:
    fullname: Y Yu
– volume: 48
  start-page: 452
  issue: 3
  year: 2013
  ident: 6320_CR18
  publication-title: Bone Marrow Transplantat
  doi: 10.1038/bmt.2012.244
  contributor:
    fullname: Y Kanda
– volume: 13
  start-page: e1441657
  issue: 4
  year: 2018
  ident: 6320_CR21
  publication-title: Plant Signal Behav
  doi: 10.1080/15592324.2018.1441657
  contributor:
    fullname: M Mookkan
– volume: 22
  start-page: 415
  issue: 5
  year: 2005
  ident: 6320_CR8
  publication-title: Plant Biotechnol
  doi: 10.5511/plantbiotechnology.22.415
  contributor:
    fullname: S Arimura
– volume: 19
  start-page: 1582
  issue: 5
  year: 2018
  ident: 6320_CR6
  publication-title: Biomacromolecules
  doi: 10.1021/acs.biomac.8b00323
  contributor:
    fullname: T Yoshizumi
– volume: 15
  start-page: 217
  issue: 4
  year: 1998
  ident: 6320_CR17
  publication-title: Plant Biotechnol
  doi: 10.5511/plantbiotechnology.15.217
  contributor:
    fullname: YY Yamamoto
– volume: 2
  start-page: 100141
  issue: 2
  year: 2021
  ident: 6320_CR4
  publication-title: Plant Commun
  doi: 10.1016/j.xplc.2021.100141
  contributor:
    fullname: S Li
– volume: 32
  start-page: 39
  year: 2015
  ident: 6320_CR15
  publication-title: Plant Biotechnol
  doi: 10.5511/plantbiotechnology.14.1210b
  contributor:
    fullname: M Lakshmanan
– volume: 98
  start-page: 4295
  issue: 8
  year: 2001
  ident: 6320_CR19
  publication-title: Proc Natl Acad Sci U S A
  doi: 10.1073/pnas.081065198
  contributor:
    fullname: DJ Stephens
– volume: 158
  start-page: 905
  issue: 2
  year: 2012
  ident: 6320_CR9
  publication-title: Plant Physiol
  doi: 10.1104/pp.111.186957
  contributor:
    fullname: M Crumpton-Taylor
– volume: 15
  start-page: 473
  issue: 3
  year: 1962
  ident: 6320_CR10
  publication-title: Physiol Plant
  doi: 10.1111/j.1399-3054.1962.tb08052.x
  contributor:
    fullname: T Murashige
– volume: 9
  start-page: 1
  issue: 1
  year: 2019
  ident: 6320_CR11
  publication-title: Sci Rep
  doi: 10.1038/s41598-018-36466-1
  contributor:
    fullname: K Midorikawa
– volume: 37
  start-page: 223
  issue: 2
  year: 2020
  ident: 6320_CR28
  publication-title: Plant Biotechnol
  doi: 10.5511/plantbiotechnology.20.0503a
  contributor:
    fullname: A Okuzaki
– volume: 2124
  start-page: 125
  year: 2020
  ident: 6320_CR27
  publication-title: Methods Mol Biol
  doi: 10.1007/978-1-0716-0356-7_6
  contributor:
    fullname: B Lacroix
SSID ssj0061881
Score 2.369599
Snippet A centrifugation-assisted peptide-mediated gene transfer (CAPT) method was recently developed as an efficient system for gene delivery into plant cells....
Abstract Objective A centrifugation-assisted peptide-mediated gene transfer (CAPT) method was recently developed as an efficient system for gene delivery into...
Objective A centrifugation-assisted peptide-mediated gene transfer (CAPT) method was recently developed as an efficient system for gene delivery into plant...
ObjectiveA centrifugation-assisted peptide-mediated gene transfer (CAPT) method was recently developed as an efficient system for gene delivery into plant...
OBJECTIVEA centrifugation-assisted peptide-mediated gene transfer (CAPT) method was recently developed as an efficient system for gene delivery into plant...
Abstract Objective A centrifugation-assisted peptide-mediated gene transfer (CAPT) method was recently developed as an efficient system for gene delivery into...
SourceID doaj
pubmedcentral
proquest
gale
crossref
pubmed
SourceType Open Website
Open Access Repository
Aggregation Database
Index Database
StartPage 46
SubjectTerms Centrifugation
Chloroplasts
Cytotoxicity
Deoxyribonucleic acid
DNA
DNA - genetics
DNA - metabolism
DNA-peptide complex
Efficiency
Flowers & plants
Gene expression
Gene transfer
Gene Transfer Techniques
Genes
Genomes
Gold
Membranes
Mitochondria
Mitochondrial DNA
Nanoluc
Particle bombardment
Particle bombardment-assisted peptide-mediated gene transfer (PBPT)
Peptides
Peptides - genetics
Permeability
Plant cells
Plant genetics
Plant mitochondria
Plants
Research Note
Seeds
Transformation, Genetic
SummonAdditionalLinks – databaseName: Directory of Open Access Journals
  dbid: DOA
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Nb9QwELVQJSQuiPIZKCggJA7IauzYjnMsiKog8XGgqDfLdhyoBNmqu3vYf983Tna1EQcu3FbxeLV-9njeZD3PjL22sk1G9J4jFkSumqS597HlyspOaY-MxVCh8Ocv5uxcfbrQF3tXfdGZsFEeeATuWMEDUvISkRKpnK5Ch6wqyN4m2yrRprz7Cr1NpsY92AhrxbZExprjpQCLURzxiSMkU83wLAxltf6_9-S9oDQ_MLkXgU7vsbsTdSxPxp98yG6l4T67PV4muXnA_LdpMGVY_AmYeHrvx8GNaSK78oqOr3SJ51IReoCVk8pV5q3pugR3LUm6-PemTFlVAp3H1vwJX-M3D9n56Yfv78_4dIECjyAqK25ThcQ5GPQTVSAF2rYTofW1CsqrgFQsIcDXoZIhGJlkiFW0AhHKhxhr39WP2MGwGNITVmptfA9f71RnlQcvhAXYku1FJzw4ZsHebvF0V6NOhsv5hTVuRN8BfZfRd3XB3hHkO0vSuM4PMPNuAsv9a-YL9oomzJGKxUDHZH769XLpPn794U4alf_vrWzB3kxG_QKgRT9VHWBUJHw1szyaWcLN4rx5uy7c5OZLR1d7gW83uinYy10z9aSja0NarGHTtNgTgb0u2ONxGe3GXTek2Ghkwexsgc2AmbcMl7-yCDiJMpGy-NP_AeUzdkdm54CPmCN2sLpep-cgW6vwIvvVDWtaJVw
  priority: 102
  providerName: Directory of Open Access Journals
– databaseName: ProQuest Central
  dbid: BENPR
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Lb9QwELagFRKXijeBggJC4oCsxo7jOCfUolYFiVIhinqz_EpBosmy2T3sv2fGcZZGSNxW8TjajMcz39jjz4S8UbwJkrWGQixwVNShosa4hgrFvagMZCwSDwp_PpOnF-LTZXWZFtyGVFY5-cToqH3vcI38AG9RAmhTV_X7xW-Kt0bh7mq6QuM22eVM4Dbt7tHx2fnXyRdLphSbjsooeTAwQDOCQpyiEJrx7PAsHEXW_n99843gNC-cvBGJTu6RvQQh88NxzO-TW6F7QO6Ml0puHhJznqwht_21BQPA9T8KGBkH1OcLLGPxgcYjI_gALCjkq4hfwzIHDJsjhfGvTR4iuwR0HlvjL3iN2TwiFyfH3z6c0nSRAnUAWFZUhQISaCuhHyssMtE2ntnGlMIKIyykZAECfWkLbq3kgVtXOMUgUhnrXGl8-ZjsdH0XnpK8qqRpYc574ZUwgA9BAlCTaplnBrBmRt5N-tSLkS9DxzxDST1qX4P2ddS-LjNyhCrfSiLXdXzQL690UpYW4ANDMBywEiTzVWE95NWWtyqoRrAmZOQ1DphGNosOy2WuzHoY9Mcv3_VhLeK-b6Ey8jYJtT0ozZl0-gC-CgmwZpL7M0mYbm7ePNmFTtN90H-NMyOvts3YE0vYutCvQaZuwDeC7quMPBnNaPvdZY3MjZJnRM0MbKaYeUv380ckA0dyJmQYf_b___Wc3OXR7MH65T7ZWS3X4QXAqZV9mebMH-rUHyQ
  priority: 102
  providerName: ProQuest
– databaseName: Scholars Portal Journals: Open Access
  dbid: M48
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3Nb9UwDLfGEGiXiW8KGyoIiQMKNGmapgc0DcQ0kAYceGi3KGnSgTTa8T6kvf8eO22fVrETt6qxK9Wx45_b5GeAl1pUQfHGMswFNZNlKJi1dcWkFl4WFisWRQeFT76o45n8fFqcbsHY7mgw4OLa0o76Sc3m528u_6wPMODfxYDX6u2CI0aRDLMPw4RLJ4JvwE0hc0kefyI3fxUU15qPB2eu1duB23lJJHpKTPJUpPP_d9G-krWmOyqvpKijO7A7YMv0sHeGu7AV2ntwq-82ub4P9tvgJqnrfjv0DPowyBA800z79IL2t_jA4lkSuoGuFdJlBLZhniK4TYnb-Hydhkg7gcr9aLzCx9j1A5gdffz-4ZgNHRZYjUhmyXTIsLJ2CvV45oiitvLcVTaXTlrpsFYLiABylwnnlAjC1VmtOaYw6-o6tz5_CNtt14bHkBaFsg0uBl56LS0CR5RAOKUb7rlFEJrA69Ge5qIn0jCxANHK9BNhcCJMnAiTJ_CeTL6RJBLseKObn5nBWEbi4hiCFQiisMovMuex4Hai0UFXklchgRc0YYZoLlraR3NmV4uF-fT1hzksZfwhnOkEXg1CTYdGq-1wLAHfipixJpJ7E0mMw3o6PPqFGd3YUO8vBORlUSbwfDNMmrS3rQ3dCmXKChdNtH2RwKPejTbvPXpjAnriYBPDTEfaXz8jSzixNhH1-JP_V30KOyJGBwaJ2oPt5XwV9hGDLd2zGFh_Ac7oLnA
  priority: 102
  providerName: Scholars Portal
Title Particle bombardment-assisted peptide-mediated gene transfer for highly efficient transient assay
URI https://www.ncbi.nlm.nih.gov/pubmed/37024962
https://www.proquest.com/docview/2803020757
https://search.proquest.com/docview/2798709225
https://pubmed.ncbi.nlm.nih.gov/PMC10080836
https://doaj.org/article/4376eea265804650bd133b2f8e89419e
Volume 16
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9swDCbaDht6Gfaety7whgE7DGr8kGX5mBYtugDpgm0dchMkWe4KNE6QxyH_fqRsBzV22yUJLCqISFH86JCfAT7LpHAirjTDWGAZz13GtLYF4zIpeaYxYxHUKDy5Flc3fDzLZgcgul4YX7Rvzd1pfT8_re_--NrK5dwOuzqx4XRyToQ0xKo8PITDPE27HL05f0UsZdy1x0gxXMeIYDjD2MQwHFO_8DE8SXOiyhNJLxp50v5_j-YHsalfN_kgEF0-g6ctggxHzS99DgeufgGPm2dK7l6CnrZrCM1ibtD-dPuPIUQme5bhkqpYSsd8xwhdwA3kwo2Hr24VIoQNicH4fhc6Ty6Bk5tR_wm_Ru9ewc3lxa_zK9Y-R4FZxCsbJl2E-bMROC-ODBHRFmVsCp1ywzU3mJE5jPOpiRJjROISYyMrYwxU2lib6jJ9DUf1onZvIcwyoSt0-ZKXkmuEhyiBoElWcRlrhJoBfO30qZYNXYbyaYYUqjGEQkMobwiVBnBGKt9LEtW1v7BY3apWWYrjEeicThAqYS6fRabEtNoklXSy4HHhAvhEBlNEZlFTtcyt3q7X6tv332qUc_-3byQD-NIKVQtUmtVt8wGuivivepInPUn0Ntsf7vaFar19regJXwi78ywP4ON-mGZSBVvtFluUyQs8GlH3WQBvmm20X3e3GwOQvQ3WU0x_BF3Dc4F3rvDu_6e-h-PEewc6iTiBo81q6z4g0tqYAbrXLB_Ao9Fo_HOM72cX19MfA3_fAl8nXA686_0FBnAs_A
link.rule.ids 230,315,733,786,790,870,891,2115,12083,21416,24346,27955,27956,31752,31753,33777,33778,43343,43838,53825,53827
linkProvider National Library of Medicine
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Lb9QwEB7BVgguiDeBAgEhcUBW48RxnBNqUasttEuFWtSbZSdOQYJk2ewe9t8z43iXRkjcongcJeN5fOPYnwHeqrR0kjeGYS6omChczoypSiZUWovcYMUiaaPw6UxOL8Sny_wyTLj1YVnlJib6QF13Fc2R79EpSghtirz4MP_N6NQo-rsajtC4CTtEuakmsHNwODv7uonFkivFN1tllNzrOaIZwTBPMUzNtHd4lI48a_-_sflachovnLyWiY7uwd0AIeP9Yczvww3XPoBbw6GS64dgzoI1xLb7ZdEAaP6PIUamAa3jOS1jqR3zW0boBlqQi5cev7pFjBg2Jgrjn-vYeXYJ7Dy0-it8jFk_goujw_OPUxYOUmAVApYlUy7BAtpK7McTS0y0Zc1taTJhhREWSzKHiT6zSWqtTF1qq6RSHDOVsVWVmTp7DJO2a91TiPNcmgZ9vha1EgbxIUogalINr7lBrBnB-40-9Xzgy9C-zlBSD9rXqH3tta-zCA5I5VtJ4rr2N7rFlQ7K0gJjoHMmRayExXye2Brraps2yqlS8NJF8IYGTBObRUvLZa7Mqu_18Zdver8Q_r9voiJ4F4SaDpVWmbD7AL-KCLBGkrsjSXS3aty8sQsd3L3Xf40zgtfbZupJS9ha161QpigxNqLu8wieDGa0_e6sIOZGmUagRgY2Usy4pf3x3ZOBEzkTMYw_-_97vYLb0_PTE31yPPv8HO6k3gXQE-QuTJaLlXuB0GppXwb_-QOnZCIa
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwEB5BEVUvvKGBAgEhcUDevBzHOZbCqgVa9kBRJQ6W7TilajdZbbKH5dczdpJVA7feongmku15fLM7_gzwjse5YVEpCeYCTWhmUiKlzgnlcUFTiRULsweFj0_Y4Sn9cpae9V2VTd9WWWl1Mamu5pPq4rfrrVzMdTD0iQWz4wNLSGNZlYNFUQa34Q46bZwNlXoXhVnEeTQckuEsaCLEMZRghiKYlO2p4R3YTjJLmMfiUU5y1P3_B-hrGWrcPXktHU3vw69hIl0XyuVk1aqJ_vMPx-PNZvoA7vUo1d_vZB7CLVM9grvdvZXrxyBnvbav6rlCG7M_MRKE4dZmCn9hO2UKQ9ypFPsCjdT4rYPIZukjTPYtS_LV2jeOwAKVu1H3hJ-R6ydwOv384-CQ9Hc1EI2YqCXchFijK4Z6Uags2W1eRCqXCVVUUoVVn0EskagwVorFJlY61DzCZCiV1okskqewVdWV2QU_TZksMawUtOBUIgRFCQRmvIyKSCKc9eDDsFti0VFyCFfKcCa6bRa4zcJts0g8-Gg3dCNp6bTdi3p5LvrFEhTDrDEyRjgWUsSsqsDSXcUlNzynUW48eGvNQVjCjMp25JzLVdOIo-8_xX5G3V_LIffgfS9U1rhoWvYHHHBWlmNrJLk3kkSP1uPhwepEH1EaYW8RQ2ifpZkHbzbDVtN2yVWmXqFMlmP4xbVPPXjWGelm3oOte8BH5jtamPEIGqXjGx-M8PnNVV_D9uzTVHw7Ovn6AnZi54bojWwPttrlyrxEYNeqV86D_wIfIUvB
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Particle+bombardment-assisted+peptide-mediated+gene+transfer+for+highly+efficient+transient+assay&rft.jtitle=BMC+research+notes&rft.au=Kimura%2C+Mitsuhiro&rft.au=Endo%2C+Akira&rft.au=Nagira%2C+Yozo&rft.au=Yoshizumi%2C+Takeshi&rft.date=2023-04-06&rft.pub=BioMed+Central&rft.eissn=1756-0500&rft.volume=16&rft_id=info:doi/10.1186%2Fs13104-023-06320-3&rft_id=info%3Apmid%2F37024962&rft.externalDBID=PMC10080836
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1756-0500&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1756-0500&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1756-0500&client=summon