Tyrosinase-Based Reporter Gene for Photoacoustic Imaging of MicroRNA-9 Regulated by DNA Methylation in Living Subjects

• Published in: Molecular therapy. Nucleic acids Vol. 11; no. C; pp. 34 - 40
• Main Authors: Zheng, Haifeng, Zhou, Lin, Shi, Yaru, Tian, Jie, Wang, Fu
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.06.2018; Elsevier Limited; American Society of Gene & Cell Therapy; Elsevier
• Subjects: Azacytidine; Bioluminescence; Cancer; Cytomegalovirus; Deoxyribonucleic acid; DNA; DNA methylation; DNA microarrays; Enzymes; Gene expression; Laboratory animals; Lysis; Melanin; MicroRNAs; miRNA; miRNAs; Northern blotting; photoacoustic imaging; Reporter gene; tyrosinase; United States > US; miRNAs; tyrosinase; reporter gene; photoacoustic imaging

MicroRNAs (miRNAs) are a class of negative regulators of gene expression and play critical roles in various biological processes. Conventional approaches for detecting miRNAs, such as northern blotting, microarray, and real-time PCR, usually require the lysis of cell samples and could not provide th...