C-terminal UBA domains protect ubiquitin receptors by preventing initiation of protein degradation

• Published in: Nature communications Vol. 2; no. 1; p. 191
• Main Authors: Heinen, Christian, Ács, Klàra, Hoogstraten, Deborah, Dantuma, Nico P.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 08.02.2011; Nature Publishing Group
• Subjects: 631/45/612/1254; 631/80/474/2085; Biodegradation; Blotting, Western; Cell Cycle Proteins - genetics; Cell Cycle Proteins - metabolism; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Electrophoresis, Polyacrylamide Gel; Flow Cytometry; Humanities and Social Sciences; multidisciplinary; Plasmids - genetics; Proteasome Endopeptidase Complex - metabolism; Proteasome Endopeptidase Complex - physiology; Protein Structure, Tertiary - genetics; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; Science; Science (multidisciplinary); Ubiquitins - genetics; Ubiquitins - metabolism

The ubiquitin receptors Rad23 and Dsk2 deliver polyubiquitylated substrates to the proteasome for destruction. The C-terminal ubiquitin-associated (UBA) domain of Rad23 functions as a cis -acting stabilization signal that protects this protein from proteasomal degradation. Here, we provide evidence...