Tailored tetravalent antibodies potently and specifically activate Wnt/Frizzled pathways in cells, organoids and mice

• Published in: eLife Vol. 8
• Main Authors: Tao, Yuyong, Mis, Monika, Blazer, Levi, Ustav, Mart, Steinhart, Zachary, Chidiac, Rony, Kubarakos, Elli, O'Brien, Siobhan, Wang, Xiaomei, Jarvik, Nick, Patel, Nish, Adams, Jarrett, Moffat, Jason, Angers, Stephane, Sidhu, Sachdev S
• Format: Journal Article
• Language: English
• Published: England eLife Science Publications, Ltd 27.08.2019; eLife Sciences Publications, Ltd; eLife Sciences Publications Ltd
• Subjects: Animals; Antibodies; Antibodies - metabolism; antibody; Biochemistry and Chemical Biology; Cell Line; Cell receptors; Cells (Biology); Developmental Biology; frizzled; Frizzled Receptors - agonists; Homeostasis; Humans; Low Density Lipoprotein Receptor-Related Protein-5 - agonists; Low Density Lipoprotein Receptor-Related Protein-6 - agonists; Mice; organoid; Organoids - drug effects; Organoids - growth & development; Physiological aspects; pluripotent stem cell; Pluripotent Stem Cells - drug effects; Pluripotent Stem Cells - physiology; Protein Binding; Proteins; regenerative medicine; Stem cells; Therapeutics; Tools and Resources; Wnt; Wnt proteins; Wnt Signaling Pathway - drug effects; Canada; mouse; antibody; chemical biology; frizzled; developmental biology; pluripotent stem cell; regenerative medicine; biochemistry; Wnt; organoid

Secreted Wnt proteins regulate development and adult tissue homeostasis by binding and activating cell-surface Frizzled receptors and co-receptors including LRP5/6. The hydrophobicity of Wnt proteins has complicated their purification and limited their use in basic research and as therapeutics. We d...