Bidirectional promoter trapping T-DNA for insertional mutagenesis in Verticillium dahliae

• Published in: Canadian journal of microbiology Vol. 60; no. 7; pp. 445 - 454
• Main Authors: Deng, Sheng, Wang, Cai-yue, Zhang, Xin, Lin, Ling
• Format: Journal Article
• Language: English
• Published: Canada NRC Research Press 01.07.2014; Canadian Science Publishing NRC Research Press
• Subjects: agar; Agrobacterium radiobacter; Agrobacterium tumefaciens; Agrobacterium tumefaciens - physiology; Agrobacterium tumefaciens-mediated transformation; Cloning; Cotton; Deoxyribonucleic acid; DNA; DNA sequencing; DNA, Bacterial - genetics; Fungi; Genes; Genetic research; Genetic Vectors; Gossypium - microbiology; Gram-negative bacteria; green fluorescent protein; gènes liés à la pathogénicité; insertional mutagenesis; Microbiological research; Microbiology; Mutagenesis; Mutagenesis, Insertional - methods; mutants; Nucleotide sequencing; pathogenicity-related genes; Pathogens; phenotype; piégeage de promoteurs; plant pathogenic fungi; promoter regions; Promoter Regions, Genetic; promoter trapping; Promoters (Genetics); Proteins; quantitative polymerase chain reaction; Real-Time Polymerase Chain Reaction; root exudates; screening; Seedlings; Seedlings - microbiology; Southern blotting; stems; transfer DNA; transformation via Agrobacterium tumefaciens; Transformation, Genetic; Trapping; UTP-glucose-1-phosphate uridylyltransferase; Verticillium - genetics; Verticillium - pathogenicity; Verticillium dahliae; virulence; Virulence - genetics; Verticillium dahliae; transformation via Agrobacterium tumefaciens; Agrobacterium tumefaciens-mediated transformation; promoter trapping; pathogenicity-related genes; gènes liés à la pathogénicité; piégeage de promoteurs

Transfer DNA (T-DNA)-based random insertional mutagenesis is a universal forward genetic approach for gene identification and cloning in many phytopathogenic fungi. In a large number of randomly selected transformants, screening for mutants with a specific phenotype is laborious, especially for path...