Bidirectional promoter trapping T-DNA for insertional mutagenesis in Verticillium dahliae - Deng, Sheng; Wang, Cai-yue; Zhang, Xin; Lin, Ling (Canadian journal of microbiology, 2014)
Keywords: agar; Agrobacterium radiobacter; Agrobacterium tumefaciens; Agrobacterium tumefaciens - physiology; Agrobacterium tumefaciens-mediated transformation; Cloning; Cotton; Deoxyribonucleic acid; DNA; DNA sequencing; DNA, Bacterial - genetics; Fungi; Genes; Genetic research; Genetic Vectors; Gossypium - microbiology; Gram-negative bacteria; green fluorescent protein; gènes liés à la pathogénicité; insertional mutagenesis; Microbiological research; Microbiology; Mutagenesis; Mutagenesis, Insertional - methods; mutants; Nucleotide sequencing; pathogenicity-related genes; Pathogens; phenotype; piégeage de promoteurs; plant pathogenic fungi; promoter regions; Promoter Regions, Genetic; promoter trapping; Promoters (Genetics); Proteins; quantitative polymerase chain reaction; Real-Time Polymerase Chain Reaction; root exudates; screening; Seedlings; Seedlings - microbiology; Southern blotting; stems; transfer DNA; transformation via Agrobacterium tumefaciens; Transformation, Genetic; Trapping; UTP-glucose-1-phosphate uridylyltransferase; Verticillium - genetics; Verticillium - pathogenicity; Verticillium dahliae; virulence; Virulence - genetics; Verticillium dahliae; transformation via Agrobacterium tumefaciens; Agrobacterium tumefaciens-mediated transformation; promoter trapping; pathogenicity-related genes; gènes liés à la pathogénicité; piégeage de promoteurs