A positive regulatory gene, THI3, is required for thiamine metabolism in Saccharomyces cerevisiae

• Published in: Journal of Bacteriology Vol. 174; no. 14; pp. 4701 - 4706
• Main Authors: Nishimura, H. (Kyoto Prefectural University of Medicine, Kamigyo-ku, Kyoto, Japan), Kawasaki, Y, Kaneko, Y, Nosaka, K, Iwashima, A
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.07.1992
• Subjects: Acid Phosphatase; Acid Phosphatase - metabolism; ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; Bacteriology; Biological and medical sciences; Biological Transport; Cloning, Molecular; drug effects; enzyme activity; Enzyme Repression; Enzyme Repression - genetics; enzymology; Ethyl Methanesulfonate; Ethyl Methanesulfonate - pharmacology; Fundamental and applied biological sciences. Psychology; GENE; GENES; Genes, Regulator; Genes, Regulator - drug effects; Genes, Regulator - genetics; Genes. Genome; Genetics; Metabolism; METABOLISME; METABOLISMO; Molecular and cellular biology; Molecular genetics; Mutagenesis; MUTANT; MUTANTES; mutants; Mutation; pharmacology; Phenotype; Pyrimidines; Pyrimidines - metabolism; SACCHAROMYCES CEREVISIAE; Saccharomyces cerevisiae - drug effects; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Subcellular Fractions; Subcellular Fractions - enzymology; thiamin; THIAMINE; Thiamine - metabolism; Thiazoles; Thiazoles - metabolism; TIAMINA; Transformation, Genetic; Yeast; Metabolism; Fungi; Regulation(control); Enzymatic activity; Gene; Membrane transport; Ascomycetes; Complementation; Mutation; Saccharomyces cerevisiae; Thiamin; Thallophyta

We have isolated a thiamine auxotrophic mutant carrying a recessive mutation which lacks the positive regulatory gene, THI3, which differs in the regulation of thiamine transport from the THI2 (PHO6) gene described previously (Y. Kawasaki, K. Nosaka, Y. Kaneko, H. Nishimura, and A. Iwashima, J. Bact...