A positive regulatory gene, THI3, is required for thiamine metabolism in Saccharomyces cerevisiae
We have isolated a thiamine auxotrophic mutant carrying a recessive mutation which lacks the positive regulatory gene, THI3, which differs in the regulation of thiamine transport from the THI2 (PHO6) gene described previously (Y. Kawasaki, K. Nosaka, Y. Kaneko, H. Nishimura, and A. Iwashima, J. Bact...
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| Published in | Journal of Bacteriology Vol. 174; no. 14; pp. 4701 - 4706 |
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| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
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Washington, DC
American Society for Microbiology
01.07.1992
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| Online Access | Get full text |
| ISSN | 0021-9193 1098-5530 1067-8832 |
| DOI | 10.1128/jb.174.14.4701-4706.1992 |
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| Abstract | We have isolated a thiamine auxotrophic mutant carrying a recessive mutation which lacks the positive regulatory gene, THI3, which differs in the regulation of thiamine transport from the THI2 (PHO6) gene described previously (Y. Kawasaki, K. Nosaka, Y. Kaneko, H. Nishimura, and A. Iwashima, J. Bacteriol. 172:6145-6147, 1990) for expression of thiamine metabolism in Saccharomyces cerevisiae. The mutant (thi3) had a markedly reduced thiamine transport system as well as reduced activity of thiamine-repressible acid phosphatase and of several enzymes for thiamine synthesis from 2-methyl-4-amino-5-hydroxymethylpyrimidine and 4-methyl-5-beta-hydroxyethylthiazole. These results suggest that thiamine metabolism in S. cerevisiae is subject to two positive regulatory genes, THI2 (PHO6) and THI3. We have also isolated a hybrid plasmid, pTTR1, containing a 6.2-kb DNA fragment from an S. cerevisiae genomic library which complements thiamine auxotrophy in the thi3 mutant. This gene was localized on a 3.0-kb ClaI-BglII fragment in the subclone pTRR5. Complementation of the activities for thiamine metabolism in the thi3 mutant transformed by some plasmids with the THI3 gene was also examined |
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| AbstractList | We have isolated a thiamine auxotrophic mutant carrying a recessive mutation which lacks the positive regulatory gene, THI3, which differs in the regulation of thiamine transport from the THI2 (PHO6) gene described previously (Y. Kawasaki, K. Nosaka, Y. Kaneko, H. Nishimura, and A. Iwashima, J. Bacteriol. 172:6145-6147, 1990) for expression of thiamine metabolism in Saccharomyces cerevisiae. The mutant (thi3) had a markedly reduced thiamine transport system as well as reduced activity of thiamine-repressible acid phosphatase and of several enzymes for thiamine synthesis from 2-methyl-4-amino-5-hydroxymethylpyrimidine and 4-methyl-5-beta-hydroxyethylthiazole. These results suggest that thiamine metabolism in S. cerevisiae is subject to two positive regulatory genes, THI2 (PHO6) and THI3. We have also isolated a hybrid plasmid, pTTR1, containing a 6.2-kb DNA fragment from an S. cerevisiae genomic library which complements thiamine auxotrophy in the thi3 mutant. This gene was localized on a 3.0-kb ClaI-BglII fragment in the subclone pTTR5. Complementation of the activities for thiamine metabolism in the thi3 mutant transformed by some plasmids with the THI3 gene was also examined. We have isolated a thiamine auxotrophic mutant carrying a recessive mutation which lacks the positive regulatory gene, THI3, which differs in the regulation of thiamine transport from the THI2 (PHO6) gene described previously (Y. Kawasaki, K. Nosaka, Y. Kaneko, H. Nishimura, and A. Iwashima, J. Bacteriol. 172:6145-6147, 1990) for expression of thiamine metabolism in Saccharomyces cerevisiae. The mutant (thi3) had a markedly reduced thiamine transport system as well as reduced activity of thiamine-repressible acid phosphatase and of several enzymes for thiamine synthesis from 2-methyl-4-amino-5-hydroxymethylpyrimidine and 4-methyl-5-beta-hydroxyethylthiazole. These results suggest that thiamine metabolism in S. cerevisiae is subject to two positive regulatory genes, THI2 (PHO6) and THI3. We have also isolated a hybrid plasmid, pTTR1, containing a 6.2-kb DNA fragment from an S. cerevisiae genomic library which complements thiamine auxotrophy in the thi3 mutant. This gene was localized on a 3.0-kb ClaI-BglII fragment in the subclone pTRR5. Complementation of the activities for thiamine metabolism in the thi3 mutant transformed by some plasmids with the THI3 gene was also examined. A thiamine auxotrophic mutant carrying a recessive mutation that lacks the positive regulatory gene, THI3, was isolated and examined. The results suggest that thiamine metabolism in Saccharomyces cerevisiae is subject to two positive regulatory genes. We have isolated a thiamine auxotrophic mutant carrying a recessive mutation which lacks the positive regulatory gene, THI3, which differs in the regulation of thiamine transport from the THI2 (PHO6) gene described previously (Y. Kawasaki, K. Nosaka, Y. Kaneko, H. Nishimura, and A. Iwashima, J. Bacteriol. 172:6145-6147, 1990) for expression of thiamine metabolism in Saccharomyces cerevisiae. The mutant (thi3) had a markedly reduced thiamine transport system as well as reduced activity of thiamine-repressible acid phosphatase and of several enzymes for thiamine synthesis from 2-methyl-4-amino-5-hydroxymethylpyrimidine and 4-methyl-5-beta-hydroxyethylthiazole. These results suggest that thiamine metabolism in S. cerevisiae is subject to two positive regulatory genes, THI2 (PHO6) and THI3. We have also isolated a hybrid plasmid, pTTR1, containing a 6.2-kb DNA fragment from an S. cerevisiae genomic library which complements thiamine auxotrophy in the thi3 mutant. This gene was localized on a 3.0-kb ClaI-BglII fragment in the subclone pTTR5. Complementation of the activities for thiamine metabolism in the thi3 mutant transformed by some plasmids with the THI3 gene was also examined.We have isolated a thiamine auxotrophic mutant carrying a recessive mutation which lacks the positive regulatory gene, THI3, which differs in the regulation of thiamine transport from the THI2 (PHO6) gene described previously (Y. Kawasaki, K. Nosaka, Y. Kaneko, H. Nishimura, and A. Iwashima, J. Bacteriol. 172:6145-6147, 1990) for expression of thiamine metabolism in Saccharomyces cerevisiae. The mutant (thi3) had a markedly reduced thiamine transport system as well as reduced activity of thiamine-repressible acid phosphatase and of several enzymes for thiamine synthesis from 2-methyl-4-amino-5-hydroxymethylpyrimidine and 4-methyl-5-beta-hydroxyethylthiazole. These results suggest that thiamine metabolism in S. cerevisiae is subject to two positive regulatory genes, THI2 (PHO6) and THI3. We have also isolated a hybrid plasmid, pTTR1, containing a 6.2-kb DNA fragment from an S. cerevisiae genomic library which complements thiamine auxotrophy in the thi3 mutant. This gene was localized on a 3.0-kb ClaI-BglII fragment in the subclone pTTR5. Complementation of the activities for thiamine metabolism in the thi3 mutant transformed by some plasmids with the THI3 gene was also examined. We have isolated a thiamine auxotrophic mutant carrying a recessive mutation which lacks the positive regulatory gene, THI3, which differs in the regulation of thiamine transport from the THI2 (PHO6) gene described previously (Y. Kawasaki, K. Nosaka, Y. Kaneko, H. Nishimura, and A. Iwashima, J. Bacteriol. 172:6145-6147, 1990) for expression of thiamine metabolism in Saccharomyces cerevisiae. The mutant (thi3) had a markedly reduced thiamine transport system as well as reduced activity of thiamine-repressible acid phosphatase and of several enzymes for thiamine synthesis from 2-methyl-4-amino-5-hydroxymethylpyrimidine and 4-methyl-5-beta-hydroxyethylthiazole. These results suggest that thiamine metabolism in S. cerevisiae is subject to two positive regulatory genes, THI2 (PHO6) and THI3. We have also isolated a hybrid plasmid, pTTR1, containing a 6.2-kb DNA fragment from an S. cerevisiae genomic library which complements thiamine auxotrophy in the thi3 mutant. This gene was localized on a 3.0-kb ClaI-BglII fragment in the subclone pTRR5. Complementation of the activities for thiamine metabolism in the thi3 mutant transformed by some plasmids with the THI3 gene was also examined Article Usage Stats Services JB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue JB About JB Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JB RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0021-9193 Online ISSN: 1098-5530 Copyright © 2014 by the American Society for Microbiology. For an alternate route to JB .asm.org, visit: JB |
| Author | Kaneko, Y Iwashima, A Kawasaki, Y Nosaka, K Nishimura, H. (Kyoto Prefectural University of Medicine, Kamigyo-ku, Kyoto, Japan) |
| AuthorAffiliation | Department of Biochemistry, Kyoto Prefectural University of Medicine, Japan |
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| Author_xml | – sequence: 1 fullname: Nishimura, H. (Kyoto Prefectural University of Medicine, Kamigyo-ku, Kyoto, Japan) – sequence: 2 fullname: Kawasaki, Y – sequence: 3 fullname: Kaneko, Y – sequence: 4 fullname: Nosaka, K – sequence: 5 fullname: Iwashima, A |
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| Cites_doi | 10.1016/0006-3002(59)90270-7 10.1128/MCB.11.6.3229 10.1002/yea.320050503 10.1016/0005-2736(75)90108-X 10.1139/g65-064 10.1016/0014-5793(92)80349-L 10.1128/MCB.5.8.2131 10.1128/JB.172.10.6145-6147.1990 10.1021/ja01523a080 10.1007/BF00330940 10.1016/0378-1119(87)90232-0 10.1016/0378-1119(79)90004-0 10.1016/S0021-9258(19)52451-6 10.1007/BF00332380 10.1128/JB.173.8.2716-2719.1991 10.1128/JB.164.2.964-968.1985 10.1007/BF00269421 10.1093/genetics/45.8.1085 10.1016/0167-4838(90)90160-H 10.1038/211206a0 10.1016/0005-2736(73)90227-7 10.1128/JB.153.1.163-168.1983 |
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| Keywords | Yeast Metabolism Fungi Regulation(control) Enzymatic activity Gene Membrane transport Ascomycetes Complementation Mutation Saccharomyces cerevisiae Thiamin Thallophyta |
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| Snippet | We have isolated a thiamine auxotrophic mutant carrying a recessive mutation which lacks the positive regulatory gene, THI3, which differs in the regulation of... Article Usage Stats Services JB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley... A thiamine auxotrophic mutant carrying a recessive mutation that lacks the positive regulatory gene, THI3, was isolated and examined. The results suggest that... |
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| SubjectTerms | Acid Phosphatase Acid Phosphatase - metabolism ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE Bacteriology Biological and medical sciences Biological Transport Cloning, Molecular drug effects enzyme activity Enzyme Repression Enzyme Repression - genetics enzymology Ethyl Methanesulfonate Ethyl Methanesulfonate - pharmacology Fundamental and applied biological sciences. Psychology GENE GENES Genes, Regulator Genes, Regulator - drug effects Genes, Regulator - genetics Genes. Genome Genetics Metabolism METABOLISME METABOLISMO Molecular and cellular biology Molecular genetics Mutagenesis MUTANT MUTANTES mutants Mutation pharmacology Phenotype Pyrimidines Pyrimidines - metabolism SACCHAROMYCES CEREVISIAE Saccharomyces cerevisiae - drug effects Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Subcellular Fractions Subcellular Fractions - enzymology thiamin THIAMINE Thiamine - metabolism Thiazoles Thiazoles - metabolism TIAMINA Transformation, Genetic |
| Title | A positive regulatory gene, THI3, is required for thiamine metabolism in Saccharomyces cerevisiae |
| URI | http://jb.asm.org/content/174/14/4701.abstract https://www.ncbi.nlm.nih.gov/pubmed/1624458 https://www.proquest.com/docview/227076104 https://www.proquest.com/docview/48341295 https://www.proquest.com/docview/73053907 https://pubmed.ncbi.nlm.nih.gov/PMC206266 |
| Volume | 174 |
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