Influenza A virus isolation, culture and identification

• Published in: Nature protocols Vol. 9; no. 11; pp. 2663 - 2681
• Main Authors: Eisfeld, Amie J, Neumann, Gabriele, Kawaoka, Yoshihiro
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.11.2014; Nature Publishing Group
• Subjects: 631/1647/1407; 631/1647/2230; 631/326/2521; 631/326/596/1578; Amplification; Analytical Chemistry; Animals; Biological assay; Biological Techniques; Cell culture; Chick Embryo - virology; Chickens; Computational Biology/Bioinformatics; Culture; Dogs; Eggs; Epidemics; Hemagglutination; Hemagglutination Tests; Hemagglutination, Viral; Identification and classification; Infection control; Influenza; Influenza A; Influenza A virus - genetics; Influenza A virus - isolation & purification; Influenza viruses; Innovations; Laboratories; Life Sciences; Madin Darby Canine Kidney Cells - virology; Mammalian cells; Mammals; Mammals - virology; Methods; Microarrays; Organic Chemistry; Pandemics; Pathogens; protocol; Reverse Transcriptase Polymerase Chain Reaction - methods; Virology - methods; Viruses; Japan

This protocol describes how to process samples potentially containing influenza A virus (IAV), amplify the samples in chicken eggs or mammalian cells and identify whether and which IAV is present. Influenza A viruses (IAVs) cause epidemics and pandemics that result in considerable financial burden a...