Expression Levels of Drug-Metabolizing Enzyme, Transporter, and Nuclear Receptor mRNAs in a Novel Three-Dimensional Culture System for Human Hepatocytes Using Micro-space Plates

• Published in: DRUG METABOLISM AND PHARMACOKINETICS Vol. 26; no. 2; pp. 137 - 144
• Main Authors: Nishimura, Masuhiro, Ejiri, Yoko, Kishimoto, Sanae, Suzuki, Satoshi, Satoh, Tetsuo, Horie, Toru, Narimatsu, Shizuo, Naito, Shinsaku
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.01.2011; Japanese Society for the Study of Xenobiotics
• Subjects: Carrier Proteins - biosynthesis; Carrier Proteins - genetics; Cell Culture Techniques - instrumentation; Cell Culture Techniques - methods; Cell Nucleus - metabolism; Cells, Cultured; drug-metabolizing enzymes; Female; Hepatocytes - enzymology; human hepatocyte; Humans; Liver - enzymology; Male; Membrane Transport Proteins - biosynthesis; Membrane Transport Proteins - genetics; micro-space plate; mRNA expression; Receptors, Cytoplasmic and Nuclear - biosynthesis; Receptors, Cytoplasmic and Nuclear - genetics; RNA, Messenger - analysis; three-dimensional culture system; transporters; three-dimensional culture system; transporters; drug-metabolizing enzymes; micro-space plate; human hepatocyte; mRNA expression

We evaluated a novel three-dimensional primary culture system using micro-space plates to determine the expression levels of 61 target (drug-metabolizing enzymes, transporters, and nuclear receptors) mRNAs in human hepatocytes. We measured mRNA expression levels of many target genes in four lots of...