原癌基因Fra-1高表达与乳腺癌细胞转移表型的关系
目的研究原癌基因Fra-1与人乳腺癌细胞系MCF-7细胞侵袭及迁移的关系。方法提取乳腺癌细胞系MDA231细胞的总RNA，通过RT—PCR扩增Fra-1全长基因，将其克隆人pcDNA3．1（-）myc-his表达载体；应用脂质体法转染MCF-7细胞，观察Fra-1过表达对MCF-7细胞增殖、黏附和迁移的影响。结果高表达Fra-1增强了MCF-7细胞的增殖、黏附和迁移的能力。结论原癌基因Fra-1可能通过促进肿瘤细胞的增殖、黏附和迁移，在肿瘤细胞的侵袭和转移过程中发挥作用。...
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| Published in | Xi'an jiao tong da xue xue bao. Journal of Xi'an Jiaotong University (medical sciences). Yi xue ban Vol. 29; no. 2; pp. 171 - 175 |
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| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
军事医学科学院基础医学研究所,北京,100850%军事医学科学院附属医院,北京,100071%军事医学科学院基础医学研究所,北京,100850
2008
青岛大学医学院附属医院肿瘤科,山东青岛,266031 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1671-8259 |
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| Abstract | 目的研究原癌基因Fra-1与人乳腺癌细胞系MCF-7细胞侵袭及迁移的关系。方法提取乳腺癌细胞系MDA231细胞的总RNA，通过RT—PCR扩增Fra-1全长基因，将其克隆人pcDNA3．1（-）myc-his表达载体；应用脂质体法转染MCF-7细胞，观察Fra-1过表达对MCF-7细胞增殖、黏附和迁移的影响。结果高表达Fra-1增强了MCF-7细胞的增殖、黏附和迁移的能力。结论原癌基因Fra-1可能通过促进肿瘤细胞的增殖、黏附和迁移，在肿瘤细胞的侵袭和转移过程中发挥作用。 |
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| AbstractList | R735.1; 目的 研究原癌基因Era-1与人乳腺癌细胞系MCF-7细胞侵袭及迁移的关系.方法 提取乳腺癌细胞系MDA231细胞的总RNA,通过RT-PCR扩增Fra-1全长基因,将其克隆入pcDNA3.1(-)mye-his表达载体;应用脂质体法转染MCF-7细胞,观察Fra-1过表达对MCF-7细胞增殖、黏附和迁移的影响.结果 高表达Fra-1增强了MCF-7细胞的增殖、黏附和迁移的能力.结论 原癌基因Fra-1可能通过促进肿瘤细胞的增殖、黏附和迁移,在肿瘤细胞的侵袭和转移过程中发挥作用. 目的研究原癌基因Fra-1与人乳腺癌细胞系MCF-7细胞侵袭及迁移的关系。方法提取乳腺癌细胞系MDA231细胞的总RNA，通过RT—PCR扩增Fra-1全长基因，将其克隆人pcDNA3．1（-）myc-his表达载体；应用脂质体法转染MCF-7细胞，观察Fra-1过表达对MCF-7细胞增殖、黏附和迁移的影响。结果高表达Fra-1增强了MCF-7细胞的增殖、黏附和迁移的能力。结论原癌基因Fra-1可能通过促进肿瘤细胞的增殖、黏附和迁移，在肿瘤细胞的侵袭和转移过程中发挥作用。 |
| Author | 宋玉华 宋三泰 江泽飞 钱露 陈立勇 郭宁 |
| AuthorAffiliation | 青岛大学医学院附属医院肿瘤科,山东青岛266031 军事医学科学院基础医学研究所,北京100850 军事医学科学院附属医院,北京100071 |
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| Author_FL | Jiang Zefei Song Yuhua Qian Lu Song Santai Guo Ning Chen Liyong |
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| SubjectTerms | AP-1 Fra-1 乳腺癌 肿瘤转移 转染 重组质粒 |
| Title | 原癌基因Fra-1高表达与乳腺癌细胞转移表型的关系 |
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