掌叶半夏主要成分对子宫颈癌细胞生长的抑制作用
目的观察掌叶半夏主要成分对子宫颈癌细胞活性、形态和超微结构、细胞周期和凋亡的影响。方法在子宫颈癌细胞SiHa的培养基中加入不同浓度的β-谷甾醇和/或掌叶半夏总蛋白培养1～7 d,采用MTT比色法检测细胞的活性。采用倒置相差显微镜、扫描和透射电镜观察细胞形态学和超微结构的变化,流式细胞术检测β-谷甾醇对细胞周期和细胞凋亡的影响。结果β-谷甾醇对子宫颈癌细胞SiHa的活性表现出明显的抑制作用,且具有时间、剂量依赖关系,掌叶半夏总蛋白对SiHa细胞的活性无显著影响。与对照组比较,20μmol/L的β-谷甾醇使SiHa细胞出现S期聚集、凋亡和坏死细胞增加,细胞形态和超微结构发生显著改变。结论掌叶半夏总...
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| Published in | Fu dan xue bao. Fudan University journal of medical science. Yi xue ban Vol. 36; no. 6; pp. 675 - 680 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
复旦大学附属妇产科医院中西医结合科,上海,200011%中科院上海生命科学院细胞资源中心,上海,200031
2009
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1672-8467 |
| DOI | 10.3969/j.issn.1672-8467.2009.06.003 |
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| Abstract | 目的观察掌叶半夏主要成分对子宫颈癌细胞活性、形态和超微结构、细胞周期和凋亡的影响。方法在子宫颈癌细胞SiHa的培养基中加入不同浓度的β-谷甾醇和/或掌叶半夏总蛋白培养1～7 d,采用MTT比色法检测细胞的活性。采用倒置相差显微镜、扫描和透射电镜观察细胞形态学和超微结构的变化,流式细胞术检测β-谷甾醇对细胞周期和细胞凋亡的影响。结果β-谷甾醇对子宫颈癌细胞SiHa的活性表现出明显的抑制作用,且具有时间、剂量依赖关系,掌叶半夏总蛋白对SiHa细胞的活性无显著影响。与对照组比较,20μmol/L的β-谷甾醇使SiHa细胞出现S期聚集、凋亡和坏死细胞增加,细胞形态和超微结构发生显著改变。结论掌叶半夏总蛋白对SiHa细胞活性的抑制作用不明显,β-谷甾醇能抑制人子宫颈癌细胞的活性,使细胞周期聚集在S期,诱导少量细胞凋亡和坏死,对细胞的形态和超微结构有显著的影响。因此,β-谷甾醇有希望成为一种安全低毒的抗子宫颈癌药物。 |
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| AbstractList | 目的观察掌叶半夏主要成分对子宫颈癌细胞活性、形态和超微结构、细胞周期和凋亡的影响。方法在子宫颈癌细胞SiHa的培养基中加入不同浓度的β-谷甾醇和/或掌叶半夏总蛋白培养1～7 d,采用MTT比色法检测细胞的活性。采用倒置相差显微镜、扫描和透射电镜观察细胞形态学和超微结构的变化,流式细胞术检测β-谷甾醇对细胞周期和细胞凋亡的影响。结果β-谷甾醇对子宫颈癌细胞SiHa的活性表现出明显的抑制作用,且具有时间、剂量依赖关系,掌叶半夏总蛋白对SiHa细胞的活性无显著影响。与对照组比较,20μmol/L的β-谷甾醇使SiHa细胞出现S期聚集、凋亡和坏死细胞增加,细胞形态和超微结构发生显著改变。结论掌叶半夏总蛋白对SiHa细胞活性的抑制作用不明显,β-谷甾醇能抑制人子宫颈癌细胞的活性,使细胞周期聚集在S期,诱导少量细胞凋亡和坏死,对细胞的形态和超微结构有显著的影响。因此,β-谷甾醇有希望成为一种安全低毒的抗子宫颈癌药物。 R711.74; 目的 观察掌叶半夏主要成分对子宫颈癌细胞活性、形态和超微结构、细胞周期和凋亡的影响.方法 在子宫颈癌细胞SiHa的培养基中加入不同浓度的β-谷甾醇和/或掌叶半夏总蛋白培养1～7 d,采用MTT比色法检测细胞的活性.采用倒置相差显微镜、扫描和透射电镜观察细胞形态学和超微结构的变化,流式细胞术检测β-谷甾醇对细胞周期和细胞凋亡的影响.结果 β-谷甾醇对子宫颈癌细胞SiHa的活性表现出明显的抑制作用,且具有时间、剂量依赖关系,掌叶半夏总蛋白对SiHa细胞的活性无显著影响.与对照组比较,20 μmol/L的β-谷甾醇使SiHa细胞出现S期聚集、凋亡和坏死细胞增加,细胞形态和超微结构发生显著改变.结论 掌叶半夏总蛋白对SiHa细胞活性的抑制作用不明显,β-谷甾醇能抑制人子宫颈癌细胞的活性,使细胞周期聚集在S期,诱导少量细胞凋亡和坏死,对细胞的形态和超微结构有显著的影响.因此,β-谷甾醇有希望成为一种安全低毒的抗子宫颈癌药物. |
| Abstract_FL | Objective To investigate the effects of Pinella's ingredients on the viability of cells, morphology, microstructure, cell cycle and apoptosis in human cervical cancer cell lines. Methods The β-sitosterol and/or total protein of Pinella were incubated at different concentrations with cervical cancer cell line SiHa. The effects of β-sitosterol and/or total protein of Pinella on the viability of cells were tested by MTT assay. The effects of β-sitosterol on morphology, microstructure, cell cycle and apoptosis were studied by phase-contrast microscope, electron microscope and flowcytometry, respectively. Results β-sitosterol could obviously inhibit the viability of SiHa cells in a time-and dose-dependent manner. The total protein of Pinella had no effects on SiHa cells' viability. 20 μmol/L β-sitosterol induced the accumulation of SiHa cells in S phase in the cell cycle. And the percents of apoptosis and necrosis increased. The morphology and microstructure of SiHa cells changed significantly after treated with 20 μmol/L β-sitosterol. Conclusions The total protein of Pinella had little influence on the viability of cervical cancer cells SiHa. The viability of SiHa cells could be suppressed by β-sitosterol. β-sitosterol could induce the accumulation of cells in S phase and the percent of apoptosis and necrosis. The morphology and microstructure changed significantly after treated with β-sitosterol. Therefore, β-sitosterol might be a prospect safe and low toxicity anti-cervical cancer drug. |
| Author | 王莉 杨永杰 归绥琪 陈松华 葛锡锐 李桂玲 |
| AuthorAffiliation | 复旦大学附属妇产科医院中西医结合科,上海200011 中科院上海生命科学院细胞资源中心,上海200031 |
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| Author_FL | CHEN Song-hua LI Gui-ling YANG Yong-jie GUI Sui-qi GE Xi-rui WANG Li |
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| ClassificationCodes | R711.74 |
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| Keywords | 细胞周期 β-谷甾醇 子宫颈癌 β-sitosterol cell cycle 掌叶半夏 Pinella cervical cancer |
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| Publisher | 复旦大学附属妇产科医院中西医结合科,上海,200011%中科院上海生命科学院细胞资源中心,上海,200031 |
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| Snippet | 目的观察掌叶半夏主要成分对子宫颈癌细胞活性、形态和超微结构、细胞周期和凋亡的影响。方法在子宫颈癌细胞SiHa的培养基中加入不同浓度的β-谷甾醇和/或掌叶半夏总蛋白培养1～7... R711.74; 目的 观察掌叶半夏主要成分对子宫颈癌细胞活性、形态和超微结构、细胞周期和凋亡的影响.方法 在子宫颈癌细胞SiHa的培养基中加入不同浓度的β-谷甾醇和/或掌叶半夏总蛋白培养1～7... |
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| Title | 掌叶半夏主要成分对子宫颈癌细胞生长的抑制作用 |
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