核转录因子激活蛋白-1对PPARδ表达的调控作用
目的研究在过氧化物酶体增长因子活化受体δ（PPARδ）表达中的相关调控因子。方法用RT-PCR扩增人类PPARδ启动子序列，通过Deletion及重组将不同长短的PPARδ启动子序列转化到PGL3-basic载体中，转染LOVO细胞，通过观测各重组体转染活性，确定PPARδ启动子活性区域。再通过电泳迁移率变更分析（EMSA）、突变等方法确定核转录因子激活蛋白-1（AP1）对PPARδ表达的作用。结果Deletion及重组转染后发现PPARδ启动子活性区域在序列3361～3464之间，EMSA、突变发现AP1因子结合位点突变后转染活性明显降低。结论AP1因子结合位点突变后转染活性明显降低，提示A...
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| Published in | Xi'an jiao tong da xue xue bao. Journal of Xi'an Jiaotong University (medical sciences). Yi xue ban Vol. 26; no. 4; pp. 312 - 315 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
西安交通大学环境与疾病相关基因教育部重点实验室,陕西西安,710061%北京大学医学部生化系,北京,853006
2005
西安交通大学医学院遗传与分子生物学系 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1671-8259 |
| DOI | 10.3969/j.issn.1671-8259.2005.04.003 |
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| Abstract | 目的研究在过氧化物酶体增长因子活化受体δ（PPARδ）表达中的相关调控因子。方法用RT-PCR扩增人类PPARδ启动子序列，通过Deletion及重组将不同长短的PPARδ启动子序列转化到PGL3-basic载体中，转染LOVO细胞，通过观测各重组体转染活性，确定PPARδ启动子活性区域。再通过电泳迁移率变更分析（EMSA）、突变等方法确定核转录因子激活蛋白-1（AP1）对PPARδ表达的作用。结果Deletion及重组转染后发现PPARδ启动子活性区域在序列3361～3464之间，EMSA、突变发现AP1因子结合位点突变后转染活性明显降低。结论AP1因子结合位点突变后转染活性明显降低，提示AP1是PPARδ的一个增强子。 |
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| AbstractList | 目的研究在过氧化物酶体增长因子活化受体δ（PPARδ）表达中的相关调控因子。方法用RT-PCR扩增人类PPARδ启动子序列，通过Deletion及重组将不同长短的PPARδ启动子序列转化到PGL3-basic载体中，转染LOVO细胞，通过观测各重组体转染活性，确定PPARδ启动子活性区域。再通过电泳迁移率变更分析（EMSA）、突变等方法确定核转录因子激活蛋白-1（AP1）对PPARδ表达的作用。结果Deletion及重组转染后发现PPARδ启动子活性区域在序列3361～3464之间，EMSA、突变发现AP1因子结合位点突变后转染活性明显降低。结论AP1因子结合位点突变后转染活性明显降低，提示AP1是PPARδ的一个增强子。 R392.11; 目的研究在过氧化物酶体增长因子活化受体δ(PPARδ)表达中的相关调控因子.方法用RT-PCR扩增人类PPARδ启动子序列,通过Deletion及重组将不同长短的PPARδ启动子序列转化到PGL3-basic载体中,转染LOVO细胞,通过观测各重组体转染活性,确定PPARδ启动子活性区域.再通过电泳迁移率变更分析(EMSA)、突变等方法确定核转录因子激活蛋白-1(AP1)对PPARδ表达的作用.结果 Deletion及重组转染后发现PPARδ启动子活性区域在序列3361～3464之间,EMSA、突变发现AP1因子结合位点突变后转染活性明显降低.结论 AP1因子结合位点突变后转染活性明显降低,提示AP1是PPARδ的一个增强子. |
| Author | 蒋晓刚 毛泽斌 孟照俊 吕晓凤 俞小瑞 |
| AuthorAffiliation | 西安交通大学医学院遗传与分子生物学系 西安交通大学环境与疾病相关基因教育部重点实验室,陕西西安710061 北京大学医学部生化系,北京853006 |
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| Author_FL | Meng Zhaojun Lü Xiaofeng Yu Xiaorui Jiang Xiaogang Mao Zebin |
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| Keywords | 突变 过氧化物酶体增长因子活化受体δ 转染 核转录因子激活蛋白-1 |
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| SubjectTerms | 核转录因子激活蛋白-1 突变 转染 过氧化物酶体增长因子活化受体δ |
| Title | 核转录因子激活蛋白-1对PPARδ表达的调控作用 |
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