Evolved Cas9 variants with broad PAM compatibility and high DNA specificity - Hu, Johnny H.; Miller, Shannon M.; Geurts, Maarten H.; Tang, Weixin; Chen, Liwei; Sun, Ning; Zeina, Christina M.; Gao, Xue; Rees, Holly A.; Lin, Zhi; Liu, David R. (Nature (London), 2018)
Keywords: 49/23; 631/1647/1511; 631/181/735; Adenine; Biological evolution; Cell division; Cloning; Compatibility; CRISPR; CRISPR-Associated Proteins - genetics; CRISPR-Associated Proteins - metabolism; CRISPR-Cas Systems - genetics; Deoxyribonucleases - metabolism; Deoxyribonucleic acid; Design; Directed Molecular Evolution; DNA; DNA - genetics; DNA - metabolism; DNA Cleavage; DNA sequencing; Editing; Evolution; Experiments; Gene disruption; Gene Editing - methods; Gene expression; Genetic aspects; Genetic variation; Genome editing; Genome, Human - genetics; Genomes; HEK293 Cells; Humanities and Social Sciences; Humans; Mammalian cells; Mammals; Methods; multidisciplinary; Mutation; Nuclease; Nucleotide Motifs; Observations; Phages; Proteins; RNA polymerase; Science; Streptococcus pyogenes; Streptococcus pyogenes - enzymology; Streptococcus pyogenes - genetics; Substrate Specificity - genetics; Transcription activation; Transcriptional Activation