Characterization of a Human Sapovirus Genotype GII.3 Strain Generated by a Reverse Genetics System: VP2 Is a Minor Structural Protein of the Virion

• Published in: Viruses Vol. 14; no. 8; p. 1649
• Main Authors: Li, Tian-Cheng, Kataoka, Michiyo, Doan, Yen Hai, Saito, Hiroyuki, Takagi, Hirotaka, Muramatsu, Masamichi, Oka, Tomoichiro
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 27.07.2022; MDPI
• Subjects: Analysis; Antigens; antiserum; carcinoma; Cell culture; Cloning; Duodenum; Genetic aspects; Genetics; genogroup GII.3; genome; Genomes; Genotype; Genotypes; Health aspects; human sapovirus; humans; HuSaV; mass spectrometry; Mass spectroscopy; Methods; Molecular weight; Nucleotide sequence; nucleotide sequences; Proteins; reverse genetics; reverse genetics system; RNA polymerase; RNA viruses; Sapovirus; Small intestine; structural proteins; Transfection; viral growth; virion; Virions; virus particle; Viruses; VP1; VP1 protein; Japan; United States > US

We devised a reverse genetics system to generate an infectious human sapovirus (HuSaV) GII.3 virus. Capped/uncapped full-length RNAs derived from HuSaV GII.3 AK11 strain generated by in vitro transcription were used to transfect HuTu80 human duodenum carcinoma cells; infectious viruses were recovere...