Sulfated glycan recognition by carbohydrate sulfatases of the human gut microbiota

• Published in: Nature chemical biology Vol. 18; no. 8; pp. 841 - 849
• Main Authors: Luis, Ana S, Baslé, Arnaud, Byrne, Dominic P., Wright, Gareth S. A., London, James A., Jin, Chunsheng, Karlsson, Niclas G., Hansson, Gunnar C., Eyers, Patrick A., Czjzek, Mirjam, Barbeyron, Tristan, Yates, Edwin A., Martens, Eric C., Cartmell, Alan
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.08.2022; Nature Publishing Group
• Subjects: 631/45/221; 631/45/535/1266; 631/45/607; Bacteria; Bacteria - metabolism; Biochemical Engineering; Biochemistry; Biochemistry, Molecular Biology; Biologi; Biological Sciences; Biology; Bioorganic Chemistry; Carbohydrates; Catabolism; Cell and Molecular Biology; Cell Biology; Cell- och molekylärbiologi; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Esters; Gastrointestinal Microbiome; Glycan; Gut microbiota; Humans; Intestinal microflora; Life Sciences; metabolism; Microbial activity; Microbiomes; Microbiota; Microorganisms; Nutrient sources; Polysaccharides; Polysaccharides - chemistry; Recognition; Structural Biology; Substrates; Sulfatases; Sulfatases - chemistry; Sulfates; Sulfates - chemistry
• ISSN: 1552-4450; 1552-4469; 1552-4469
• DOI: 10.1038/s41589-022-01039-x

Sulfated glycans are ubiquitous nutrient sources for microbial communities that have coevolved with eukaryotic hosts. Bacteria metabolize sulfated glycans by deploying carbohydrate sulfatases that remove sulfate esters. Despite the biological importance of sulfatases, the mechanisms underlying their ability to recognize their glycan substrate remain poorly understood. Here, we use structural biology to determine how sulfatases from the human gut microbiota recognize sulfated glycans. We reveal seven new carbohydrate sulfatase structures spanning four S1 sulfatase subfamilies. Structures of S1_16 and S1_46 represent novel structures of these subfamilies. Structures of S1_11 and S1_15 demonstrate how non-conserved regions of the protein drive specificity toward related but distinct glycan targets. Collectively, these data reveal that carbohydrate sulfatases are highly selective for the glycan component of their substrate. These data provide new approaches for probing sulfated glycan metabolism while revealing the roles carbohydrate sulfatases play in host glycan catabolism. Comprehensive structural biology analysis of seven members of the S1 carbohydrate sulfatase family derived from human gut microbiome Bacteroides reveals mechanisms of glycan recognition and sulfate hydrolysis.