Digital PCR assay detection of circulating Mycobacterium tuberculosis DNA in pulmonary tuberculosis patient plasma

• Published in: Tuberculosis Vol. 99; pp. 47 - 53
• Main Authors: Ushio, Ryota, Yamamoto, Masaki, Nakashima, Kentaro, Watanabe, Hiroki, Nagai, Kenjiro, Shibata, Yuji, Tashiro, Ken, Tsukahara, Toshinori, Nagakura, Hideyuki, Horita, Nobuyuki, Sato, Takashi, Shinkai, Masaharu, Kudo, Makoto, Ueda, Atsuhisa, Kaneko, Takeshi
• Format: Journal Article
• Language: English
• Published: Scotland Elsevier Ltd 01.07.2016; Elsevier BV
• Subjects: Adult; Aged; Aged, 80 and over; Case-Control Studies; Circulating DNA; Digital PCR; DNA Copy Number Variations; DNA Gyrase; DNA Gyrase - genetics; DNA, Bacterial; DNA, Bacterial - blood; Female; Gene Dosage; Genetic Markers; gyrB; Humans; Infectious Disease; IS6110; Male; Middle Aged; Mycobacterium tuberculosis; Mycobacterium tuberculosis - genetics; Plasma; Polymerase Chain Reaction; Polymerase Chain Reaction - methods; Predictive Value of Tests; Pulmonary/Respiratory; Reproducibility of Results; Tuberculosis; Tuberculosis, Pulmonary; Tuberculosis, Pulmonary - blood; Tuberculosis, Pulmonary - diagnosis; Tuberculosis, Pulmonary - microbiology; Young Adult; IS6110; Plasma; gyrB; Digital PCR; Tuberculosis; Circulating DNA

Nucleic acid amplification tests are a major diagnostic tool for pulmonary tuberculosis (PTB). Recently, digital PCR (dPCR) assay has improved sensitivity for the detection of small copy numbers of target molecules. The aim of this study is to explore the utility of dPCR for detecting Mycobacterium...