A systematic screen for protein–lipid interactions in Saccharomyces cerevisiae

• Published in: Molecular systems biology Vol. 6; no. 1; pp. 430 - n/a
• Main Authors: Gallego, Oriol, Betts, Matthew J, Gvozdenovic‐Jeremic, Jelena, Maeda, Kenji, Matetzki, Christian, Aguilar‐Gurrieri, Carmen, Beltran‐Alvarez, Pedro, Bonn, Stefan, Fernández‐Tornero, Carlos, Jensen, Lars Juhl, Kuhn, Michael, Trott, Jamie, Rybin, Vladimir, Müller, Christoph W, Bork, Peer, Kaksonen, Marko, Russell, Robert B, Gavin, Anne‐Claude
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 30.11.2010; John Wiley & Sons, Ltd; EMBO Press; Nature Publishing Group; Springer Nature
• Subjects: Actin; Algorithms; Baking yeast; Binding; E coli; EMBO20; EMBO37; Enzymes; Fatty Acid-Binding Proteins - analysis; Fatty Acid-Binding Proteins - chemistry; Fatty Acid-Binding Proteins - metabolism; High-Throughput Screening Assays - methods; Homology; interactome; Kinases; Lipid Metabolism - physiology; Lipid-Linked Proteins - analysis; Lipid-Linked Proteins - chemistry; Lipid-Linked Proteins - metabolism; Lipids; Lipids - analysis; lipid‐array; Membranes; Metabolism; Metabolites; Metabolome; Mode of action; Models, Biological; network; Phosphates; Phosphatidylinositol; Phosphatidylinositol phosphates; Pleckstrin; pleckstrin homology domains; Protein Array Analysis - methods; Protein Binding; Protein Interaction Domains and Motifs - physiology; Proteins; Saccharomyces cerevisiae; Saccharomyces cerevisiae - chemistry; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - analysis; Saccharomyces cerevisiae Proteins - chemistry; Saccharomyces cerevisiae Proteins - metabolism; Sphingolipids; Validation Studies as Topic; Yeast; pleckstrin homology domains; interactome; lipid‐array; sphingolipids; network

Protein–metabolite networks are central to biological systems, but are incompletely understood. Here, we report a screen to catalog protein–lipid interactions in yeast. We used arrays of 56 metabolites to measure lipid‐binding fingerprints of 172 proteins, including 91 with predicted lipid‐binding d...