隆朋对手术犬免疫功能的抑制作用

目的探讨隆朋麻醉、手术应激对犬机体免疫功能的影响。方法选取健康犬14只,随机分为单纯隆朋麻醉组（A组,n=7）和隆朋麻醉手术组（B组,n=7）,A组按3.0mg/kg肌肉注射隆朋进行麻醉,B组在A组基础上进行腹腔探查术,观察两组麻醉前（0h）和麻醉后2、8、24、72、120和168h血清IL-1β、IL-2、IL-4、IL-8、TNF-α含量的变化。结果与麻醉前相比,A组犬在麻醉后2～72h期间血清IL-1β、IL-2、TNF-α水平显著降低（P〈0.05）,并且在120～168h期间逐渐恢复至麻醉前水平,而麻醉后血清IL-4、IL-8水平无明显变化（P〉0.05）。与麻醉前相比,B组犬在麻...

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Published in西安交通大学学报（医学版） Vol. 38; no. 2; pp. 314 - 316
Main Author 郭健 南勇善
Format Journal Article
LanguageChinese
Published 延边大学附属医院麻醉科,吉林延吉,133000 2017
Subjects
免疫功能
手术应激
犬
细胞因子
隆朋
cytokine
Rompum
免疫功能
immune function
隆朋
细胞因子
犬
operation stress
手术应激
dog
Online AccessGet full text
ISSN1671-8259
DOI10.7652/jdyxb201702030

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Summary:目的探讨隆朋麻醉、手术应激对犬机体免疫功能的影响。方法选取健康犬14只,随机分为单纯隆朋麻醉组（A组,n=7）和隆朋麻醉手术组（B组,n=7）,A组按3.0mg/kg肌肉注射隆朋进行麻醉,B组在A组基础上进行腹腔探查术,观察两组麻醉前（0h）和麻醉后2、8、24、72、120和168h血清IL-1β、IL-2、IL-4、IL-8、TNF-α含量的变化。结果与麻醉前相比,A组犬在麻醉后2～72h期间血清IL-1β、IL-2、TNF-α水平显著降低（P〈0.05）,并且在120～168h期间逐渐恢复至麻醉前水平,而麻醉后血清IL-4、IL-8水平无明显变化（P〉0.05）。与麻醉前相比,B组犬在麻醉后2～168h期间血清IL-1β、IL-2水平显著降低（P〈0.05）,而血清IL-8、TNF-α水平显著升高（P〈0.05）。与A组麻醉后同一时间点相比,B组犬在2、8、24、72、120、168h时血清IL-1β、IL-2水平显著降低（P〈0.05）,而IL-8、TNF-α水平显著升高（P〈0.05）。结论隆朋麻醉、手术创伤应激反应均可导致犬免疫功能抑制,并且手术的影响要大于麻醉。
Bibliography:GUO Jian, NAN Yong-shan （Department of Anesthesia, the Affiliated Hospital of Yanbian University, Yanji 133000, China）
Rompum;dog;operation stress;immune function;cytokine
Objective To study the inhibitory effect of Rompun anesthesia and operation on immune function of dogs.Methods We randomly divided 14 healthy dogs into Group A and Group B with 7 in each.Rompun anesthesia induced by intramuscular injection of 3.0mg/kg Rompun was carried out in Group A while Rompun anesthesia and operation were applied in Group B.The venous blood samples were collected before and after anesthesia at 2,8,24,72,120 and 168 h for centrifugation to obtain sera.ELISA kit was used to evaluate the contents of serum IL-1β,IL-2,IL-4,IL-8 and TNF-α.Results Compared with those before anesthesia,the concentrations of IL-2,IL-1βand TNF-αin Group A significantly decreased at 2,8,24 and 72 h（P〈0.05）and resumed the pre-anesthesia level from120 to 168 h;however,the concentrations of IL-4 and IL-8 did not change significantly during 2 to 168 h（
ISSN:1671-8259
DOI:10.7652/jdyxb201702030