Muconic acid production from glucose and xylose in Pseudomonas putida via evolution and metabolic engineering

• Published in: Nature communications Vol. 13; no. 1; pp. 4925 - 14
• Main Authors: Ling, Chen, Peabody, George L., Salvachúa, Davinia, Kim, Young-Mo, Kneucker, Colin M., Calvey, Christopher H., Monninger, Michela A., Munoz, Nathalie Munoz, Poirier, Brenton C., Ramirez, Kelsey J., St. John, Peter C., Woodworth, Sean P., Magnuson, Jon K., Burnum-Johnson, Kristin E., Guss, Adam M., Johnson, Christopher W., Beckham, Gregg T.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 22.08.2022; Nature Publishing Group; Nature Portfolio
• Subjects: 09 BIOMASS FUELS; 3-Dehydroquinate synthase; 631/326/2522; 631/61/252/318; 631/61/318; Acid production; Acids; applied microbiology; Carbohydrates; Engineering; Evolution; Glucose; Humanities and Social Sciences; Hydrolysates; Laboratories; Lignocellulose; Metabolic engineering; Metabolism; Muconic acid; multidisciplinary; Mutation; performance advantaged bioproducts; Petrochemicals; Pseudomonas putida; Science; Science (multidisciplinary); shikimate; Sugar; Xylose; Xylose isomerase

Muconic acid is a bioprivileged molecule that can be converted into direct replacement chemicals for incumbent petrochemicals and performance-advantaged bioproducts. In this study, Pseudomonas putida KT2440 is engineered to convert glucose and xylose, the primary carbohydrates in lignocellulosic hyd...