Dose-Response Resistance to HIV-1/MuLV Pseudotype Virus ex vivo in a Hairpin Ribozyme Transgenic Mouse Model

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 96; no. 22; pp. 12749 - 12753
• Main Authors: Andäng, Michael, Hinkula, Jorma, Hotchkiss, Graham, Larsson, Sten, Britton, Sven, Wong-Staal, Flossie, Wahren, Britta, Ahrlund-Richter, Lars
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 26.10.1999; National Acad Sciences; National Academy of Sciences; The National Academy of Sciences
• Subjects: AIDS/HIV; Animals; Antigens; Base Sequence; Biological Sciences; Cell lines; Cytomegalovirus - genetics; Disease Models, Animal; DNA Primers; Dose-Response Relationship, Drug; Drug Resistance, Microbial; Gene therapy; HIV; HIV 1; HIV-1 - drug effects; Human immunodeficiency virus; Human immunodeficiency virus 1; Infections; Leukemia Virus, Murine - drug effects; Medicin och hälsovetenskap; Mice; Mice, Transgenic; Oligonucleotides, Antisense - pharmacology; Ribonucleic acid; ribozymes; RNA; RNA, Catalytic - genetics; Rodents; Spleen; Spleen cells; T lymphocytes; Transgenic animals; Viruses

We have investigated the efficacy of a hairpin ribozyme targeting the 5′ leader sequence of HIV-1 RNA in a transgenic model system. Primary spleen cells derived from transgenic or control mice were infected with HIV-1/MuLV pseudotype virus. A significantly reduced susceptibility to infection in ribo...