Molecular biological identification of Babesia, Theileria, and Anaplasma species in cattle in Egypt using PCR assays, gene sequence analysis and a novel DNA microarray

• Published in: Veterinary parasitology Vol. 207; no. 3-4; pp. 329 - 334
• Main Authors: El-Ashker, Maged, Hotzel, Helmut, Gwida, Mayada, El-Beskawy, Mohamed, Silaghi, Cornelia, Tomaso, Herbert
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 30.01.2015
• Subjects: Anaplasma; Anaplasma - genetics; Anaplasma - isolation & purification; Anaplasma marginale; anaplasmosis; Anaplasmosis - diagnosis; Anaplasmosis - parasitology; Anaplasmosis - pathology; Animals; Babesia; Babesia - genetics; Babesia - isolation & purification; Babesia bigemina; Babesia bovis; babesiosis; Babesiosis - diagnosis; Babesiosis - parasitology; Babesiosis - pathology; blood; blood sampling; Bovine piroplasms; carrier state; Cattle; Cattle Diseases - diagnosis; Cattle Diseases - parasitology; Cattle Diseases - pathology; clinical examination; DNA microarrays; Egypt; feces; hemoglobinuria; Microarray; microscopy; mixed infection; nucleotide sequences; Oligonucleotide Array Sequence Analysis; parasites; PCR; Piroplasmia; Polymerase Chain Reaction; Reproducibility of Results; sequence analysis; Species Specificity; Theileria; Theileria - genetics; Theileria - isolation & purification; Theileriasis - diagnosis; Theileriasis - parasitology; Theileriasis - pathology; Egypt; Bovine piroplasms; Anaplasma; Microarray; Cattle; PCR; Babesia

•A DNA microarray proved to be suitable for identification and differentiation of Babesia spp. in cattle in Egypt.•Clinical cases of acute infections with Piroplasmidae were diagnosed correctly by microscopy in most of the cases.•Cattle with babesiosis were likely develop haemoglobinuria; while thos...