Multi-institutional external validation of urinary TWIST1 and NID2 methylation as a diagnostic test for bladder cancer

• Published in: Urologic oncology Vol. 33; no. 9; pp. 387.e1 - 387.e6
• Main Authors: Fantony, Joseph J., Abern, Michael R., Gopalakrishna, Ajay, Owusu, Richmond, Jack Tay, Kae, Lance, Raymond S., Inman, Brant A.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.09.2015
• Subjects: Aged; Area Under Curve; Biomarkers, Tumor - analysis; Carcinoma, Transitional Cell - diagnosis; Carcinoma, Transitional Cell - genetics; Carcinoma, Transitional Cell - urine; Cell Adhesion Molecules - genetics; Cell Adhesion Molecules - metabolism; Cell Adhesion Molecules - urine; Cohort Studies; Diagnostic test; DNA Methylation; Epigenetic; Female; Humans; Likelihood Functions; Male; Middle Aged; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; Nuclear Proteins - urine; Real-Time Polymerase Chain Reaction; ROC Curve; Sensitivity; Sensitivity and Specificity; Smoking; Specificity; Twist-Related Protein 1 - genetics; Twist-Related Protein 1 - metabolism; Twist-Related Protein 1 - urine; Urinary Bladder Neoplasms - diagnosis; Urinary Bladder Neoplasms - genetics; Urinary Bladder Neoplasms - urine; Urology; Urothelial carcinoma; Diagnostic test; Sensitivity; Specificity; Epigenetic; Urothelial carcinoma; Smoking

We previously reported a clinical trial in which we were unable to replicate the excellent diagnostic metrics produced in the developmental study of the TWIST1 and NID2 gene methylation assay. In this expanded trial with subjects enrolled from another institution, we reexamine the diagnostic capabil...